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Annexin IV Antibody (4): sc-135831

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Datasheets
  • Annexin IV Antibody (4) is a mouse monoclonal IgG1 provided at 50 µg/0.5 ml
  • raised against amino acids 1-319 representing full length Annexin IV of human origin
  • recommended for detection of Annexin IV of mouse, rat, human and canine origin by WB, IP and IF
  • At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for Annexin IV Antibody (4). This work is in progress.

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    Annexin IV Antibody (4) is a mouse monoclonal IgG1 antibody that detects Annexin IV in mouse, rat, human, and canine samples through applications such as western blotting (WB), immunoprecipitation (IP), and immunofluorescence (IF). Annexin IV, also known as endonexin, plays a crucial role in regulating chloride ion flux within cells by mediating the activity of calmodulin kinase II (CaMKII), which is essential for various cellular processes including muscle contraction, neurotransmitter release, and cell signaling. Anti-Annexin IV antibody (4) recognizes a unique structure, characterized by a conserved core domain that binds phospholipids in a calcium-dependent manner, allowing participation in diverse cellular functions such as endocytosis, exocytosis, and cellular adhesion. Annexin IV monoclonal antibody (4) serves as an invaluable tool for researchers exploring the intricate roles of this protein in cellular physiology and pathology.

    For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

    Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA

    LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences

    Annexin IV Antibody (4) References:

    1. Characterization of the Ca2+-dependent binding of annexin IV to surfactant protein A.  |  Sohma, H., et al. 1999. Biochem J. 341 (Pt 1): 203-9. PMID: 10377263
    2. A potential endogenous ligand of annexin IV in the exocrine pancreas. Carbohydrate structure of GP-2, a glycosylphosphatidylinositol-anchored glycoprotein of zymogen granule membranes.  |  Tsujii-Hayashi, Y., et al. 2002. J Biol Chem. 277: 47493-9. PMID: 12324456
    3. Ethanol-induced augmentation of annexin IV in cultured cells and the enhancement of cytotoxicity by overexpression of annexin IV by ethanol.  |  Ohkawa, H., et al. 2002. Biochim Biophys Acta. 1588: 217-25. PMID: 12393176
    4. Augmentation of ethanol-induced cell damage and activation of nuclear factor-kappa B by annexin IV in cultured cells.  |  Sohma, H., et al. 2003. Alcohol Clin Exp Res. 27: 64S-67S. PMID: 12960510
    5. Levels of annexin IV and V in the plasma of pregnant and postpartum women.  |  Masuda, J., et al. 2004. Thromb Haemost. 91: 1129-36. PMID: 15175799
    6. Pathogenic natural antibodies recognizing annexin IV are required to develop intestinal ischemia-reperfusion injury.  |  Kulik, L., et al. 2009. J Immunol. 182: 5363-73. PMID: 19380783
    7. Wild-type p53 enhances annexin IV gene expression in ovarian clear cell adenocarcinoma.  |  Masuishi, Y., et al. 2011. FEBS J. 278: 1470-83. PMID: 21348943
    8. Annexin IV inhibits calmodulin-dependent protein kinase II-activated chloride conductance. A novel mechanism for ion channel regulation.  |  Chan, HC., et al. 1994. J Biol Chem. 269: 32464-8. PMID: 7798247
    9. A role for annexin IV in epithelial cell function. Inhibition of calcium-activated chloride conductance.  |  Kaetzel, MA., et al. 1994. J Biol Chem. 269: 5297-302. PMID: 8106514
    10. The relationship between the binding of ATP and calcium to annexin IV. Effect of nucleotide on the calcium-dependent interaction of annexin with phosphatidylserine.  |  Bandorowicz-Pikula, J., et al. 1997. Mol Membr Biol. 14: 179-86. PMID: 9491369

    Ordering Information

    Product NameCatalog #UNITPriceQtyFAVORITES

    Annexin IV Antibody (4)

    sc-135831
    50 µg/0.5 ml
    $316.00

    I am using Annexin IV (4): sc-135831 for immunofluorescence, what dilution of this antibody should I use?

    Asked by: TinTin
    Thank you for your question. We recommend a starting dilution of 1:50. However, optimal antibody concentration should be determined by titration. Please find our full immunofluorescence protocol here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
    Answered by: Technical Support
    Date published: 2017-03-24
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    Rated 4 out of 5 by from Produced great immunofluorescence nuclearProduced great immunofluorescence nuclear and cytoplasmic staining in WI-38 cells. -SCBT QC
    Date published: 2015-01-14
    Rated 4 out of 5 by from Positive detection of endogenous AnnexinPositive detection of endogenous Annexin IV in human endothelial whole cell lysate by WB. -SCBT QC
    Date published: 2015-01-03
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    Annexin IV Antibody (4) is rated 4.0 out of 5 by 2.
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