Annexin-2/ANXA2 Antibody (D1/274.5): sc-65873

Annexin II Antibody (D1/274.5) is a mouse monoclonal IgG2a kappa light chain antibody raised against AG1523 fibroblasts of human origin, capable of detecting Annexin II from mouse, rat, and human origins by western blotting (WB), immunoprecipitation (IP), and immunofluorescence (IF). Annexin II, also known as Annexin A2, Calpactin I heavy chain, Chromobindin-8, Lipocortin II, and p36, is a calcium-dependent phospholipid-binding protein involved in various cellular processes such as membrane trafficking, endocytosis, exocytosis, and cellular adhesion. A significant aspect of Annexin II is its post-translational modifications, particularly phosphorylation on tyrosine and serine residues, which are crucial for regulating its activity, localization, and interaction with other proteins. These modifications affect its ability to form heterotetramers with S100A10 (p11), known as calpactin I, influencing signal transduction pathways and cytoskeletal organization. Annexin II serves as a substrate for protein kinase C (PKC) and Src family kinases like Src pp60, highlighting its role in phosphorylation-dependent signaling mechanisms. Understanding these modifications is important because they modulate the protein′s function in processes like cell proliferation, apoptosis, and response to stress, which are vital in physiological and pathological conditions such as cancer progression and inflammation. Anti-Annexin II antibody (D1/274.5) allows researchers to investigate the protein′s role and its post-translational modifications in cellular functions and disease states. Annexin II (D1/274.5) antibody is available in both unconjugated and multiple conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and various Alexa Fluor® conjugates, providing versatility for a wide range of experimental applications.

The preferred Annexin-2/ANXA2 antibody is Annexin-2/ANXA2 Antibody (C-10): sc-28385.