Ankyrin G Antibody (H-4) recommended for detection of Ankyrin G of human origin by WB, IP, IF and ELISA

Ankyrin G Antibody (H-4): sc-166602

Ankyrin G Antibody (H-4) is rated 5.0 out of 5 by 2.
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Datasheets

    • Ankyrin G Antibody (H-4) is a mouse monoclonal IgG2b (kappa light chain) provided at 200 µg/ml
    • raised against amino acids 4163-4377 mapping at the C-terminus of Ankyrin G of human origin
    • recommended for detection of Ankyrin G of human origin by WB, IP, IF and ELISA
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • See Ankyrin G (463): sc-12719 for Ankyrin G antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.

What is the recommended starting dilution for Western blot for sc-166602: Ankyrin G (H-4) monoclonal antibody?

Asked by: Cweed
The recommended starting dilution for Western blot is 1:100 with a dilution range of 1:100-1:1000. This information, along with other technical specifications, is available in the recommended protocol: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-03-22
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Rated 5 out of 5 by from Produced positive Western Blot data of Ankyrin Produced positive Western Blot data of Ankyrin G expression in IMR-32 and Caco-2 whole cell lysates. -SCBT QC
Date published: 2015-01-13
Rated 5 out of 5 by from Produced positive Western Blot data of Ankyrin Produced positive Western Blot data of Ankyrin G expression in human skeletal muscle tissue extract. -SCBT QC
Date published: 2014-06-04
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