Angptl4 Antibody (A-2): sc-373761

Angptl4 Antibody (A-2) is a mouse monoclonal IgG1 kappa light chain antibody that detects Angptl4 of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-Angptl4 antibody (A-2) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Angiopoietin-like 4 (Angptl4), also known as hepatic fibrinogen/angiopoietin-related protein (HFARP), is a secreted protein that plays a crucial role in maintaining endothelial cell integrity and function, particularly under conditions of hypoxia. Angptl4 is induced in endothelial cells during hypoxic stress, highlighting Angptl4′s importance in vascular biology and response to oxygen deprivation. Structurally, Angptl4 features a hydrophobic N-terminal region characteristic of a secretory signal sequence, along with a consensus glycosylation site that may influence stability and activity. Notably, Angptl4 serves as a potential biomarker for renal cell carcinoma, as Angptl4 is uniquely expressed in these tumor cells, providing insights into tumor biology and potential therapeutic targets. Additionally, Angptl4 regulates glucose homeostasis, lipid metabolism, and insulin sensitivity, making Angptl4 a significant factor in metabolic disorders such as diabetes.

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Angptl4 Antibody (A-2) References:

1. Hepatic expression, synthesis and secretion of a novel fibrinogen/angiopoietin-related protein that prevents endothelial-cell apoptosis.  |  Kim, I., et al. 2000. Biochem J. 346 Pt 3: 603-10. PMID: 10698685
2. Anti-tumor effect of recombinant retroviral vector-mediated human ANGPTL4 gene transfection.  |  Li, KQ., et al. 2004. Chin Med J (Engl). 117: 1364-9. PMID: 15377430
3. Inhibition of cardiac lipoprotein utilization by transgenic overexpression of Angptl4 in the heart.  |  Yu, X., et al. 2005. Proc Natl Acad Sci U S A. 102: 1767-72. PMID: 15659544
4. Angiopoietin-like protein 4 decreases blood glucose and improves glucose tolerance but induces hyperlipidemia and hepatic steatosis in mice.  |  Xu, A., et al. 2005. Proc Natl Acad Sci U S A. 102: 6086-91. PMID: 15837923
5. Differential regulation and properties of angiopoietin-like proteins 3 and 4.  |  Ge, H., et al. 2005. J Lipid Res. 46: 1484-90. PMID: 15863837
6. ANGPTL4 in Metabolic and Cardiovascular Disease.  |  Aryal, B., et al. 2019. Trends Mol Med. 25: 723-734. PMID: 31235370
7. A TAZ-ANGPTL4-NOX2 Axis Regulates Ferroptotic Cell Death and Chemoresistance in Epithelial Ovarian Cancer.  |  Yang, WH., et al. 2020. Mol Cancer Res. 18: 79-90. PMID: 31641008
8. Hepatocyte-specific suppression of ANGPTL4 improves obesity-associated diabetes and mitigates atherosclerosis in mice.  |  Singh, AK., et al. 2021. J Clin Invest. 131: PMID: 34255741
9. Regulation of lipoprotein metabolism by ANGPTL3, ANGPTL4, and ANGPTL8.  |  Sylvers-Davie, KL. and Davies, BSJ. 2021. Am J Physiol Endocrinol Metab. 321: E493-E508. PMID: 34338039
10. STAT3 induces breast cancer growth via ANGPTL4, MMP13 and STC1 secretion by cancer associated fibroblasts.  |  Avalle, L., et al. 2022. Oncogene. 41: 1456-1467. PMID: 35042959
11. ANGPTL4 stabilizes atherosclerotic plaques and modulates the phenotypic transition of vascular smooth muscle cells through KLF4 downregulation.  |  Cho, DI., et al. 2023. Exp Mol Med. 55: 426-442. PMID: 36782020
12. ANGPTL4 accelerates ovarian serous cystadenocarcinoma carcinogenesis and angiogenesis in the tumor microenvironment by activating the JAK2/STAT3 pathway and interacting with ESM1.  |  Li, YK., et al. 2024. J Transl Med. 22: 46. PMID: 38212795

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Angptl4 Antibody (A-2) has 2 citations in a variety of scientific publications.

Primary antibodies like Anti-Angptl4 Antibody (A-2) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Angptl4 Antibody (A-2) Specifications

Alternate Product Name: Angiopoietin Like 4

Product Clone Name: A-2

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 51-250

Epitope Target: Angptl4

Epitope Species: human

Product Reactivity/Detection: Angptl4

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 2 citations in various scientific publications

Available Conjugates/Forms for Angptl4 Antibody (A-2): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred Angptl4 antibody is Angptl4 Antibody (C-7): sc-373762.