Angiopoietin 1/Ang-1/ANGPT1 Antibody (D-6): sc-518157
- Angiopoietin 1/Ang-1/ANGPT1 Antibody (D-6) is a mouse monoclonal IgG2a κ provided at 200 µg/ml
- raised against amino acids 400-498 of Ang-1 of human origin
- recommended for detection of Ang-1 of human origin by WB, IP, IF and ELISA; may cross-react with Ang-2 and Ang-4
- Anti-Angiopoietin 1/Ang-1/ANGPT1 Antibody (D-6) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgGκ BP-HRP is the preferred secondary detection reagent for Angiopoietin 1/Ang-1/ANGPT1 Antibody (D-6) for WB applications. This reagent is now offered in a bundle with Angiopoietin 1/Ang-1/ANGPT1 Antibody (D-6) (see ordering information below). For additional m-IgGκ BP conjugates see our complete list of Mouse IgG Binding Proteins.
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SEE ALSO...
Ang-1 Antibody (D-6) is a mouse monoclonal IgG2a kappa light chain antibody engineered to detect Angiopoietin-1 (Ang-1), also known as ANGPT1, of human origin through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Ang-1 monoclonal antibody (D-6) targets an epitope spanning amino acids 400-498 of human Ang-1 and exhibits potential cross-reactivity with Angiopoietin-2 (Ang-2) and Angiopoietin-4 (Ang-4), enhancing utility in various research settings. Angiopoietin-1 is a secreted protein localized in the extracellular matrix, where binding to Tie-2 receptor tyrosine kinase on endothelial and hematopoietic cells plays a crucial role in blood vessel maturation and stability. The extracellular location of Ang-1 is essential for function in angiogenesis, facilitating interactions that promote vascular integrity and remodeling. Structurally, Ang-1 features a fibrinogen-like domain at the C-terminus and coiled-coil regions at the N-terminus, vital for dimerization and effective receptor binding. Post-translational modifications, including glycosylation, are critical for stability and secretion of Ang-1, thereby influencing biological activity and interaction with receptors. Angiopoietin-1 interacts with key proteins such as Tie-2 receptor and acts antagonistically to Angiopoietin-2, modulating signaling pathways that govern endothelial cell function and vascular homeostasis. These interactions and modifications underscore Ang-1′s importance in angiogenic processes and implications in pathological conditions like cancer, where notable expression occurs in human malignant glioma. Available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates, anti-Ang-1 antibody (D-6) provides researchers with a versatile tool for investigating Angiopoietin-1-mediated signaling pathways and vascular biology impact.
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Angiopoietin 1/Ang-1/ANGPT1 Antibody (D-6) | sc-518157 | 200 µg/ml | $316.00 | |||
Angiopoietin 1/Ang-1/ANGPT1 Antibody (D-6): m-IgGκ BP-HRP Bundle | sc-542721 | 200 µg Ab; 40 µg BP | $354.00 | |||
Angiopoietin 1/Ang-1/ANGPT1 Antibody (D-6) AC | sc-518157 AC | 500 µg/ml, 25% agarose | $416.00 | |||
Angiopoietin 1/Ang-1/ANGPT1 Antibody (D-6) HRP | sc-518157 HRP | 200 µg/ml | $316.00 | |||
Angiopoietin 1/Ang-1/ANGPT1 Antibody (D-6) FITC | sc-518157 FITC | 200 µg/ml | $330.00 | |||
Angiopoietin 1/Ang-1/ANGPT1 Antibody (D-6) PE | sc-518157 PE | 200 µg/ml | $343.00 | |||
Angiopoietin 1/Ang-1/ANGPT1 Antibody (D-6) Alexa Fluor® 488 | sc-518157 AF488 | 200 µg/ml | $357.00 | |||
Angiopoietin 1/Ang-1/ANGPT1 Antibody (D-6) Alexa Fluor® 546 | sc-518157 AF546 | 200 µg/ml | $357.00 | |||
Angiopoietin 1/Ang-1/ANGPT1 Antibody (D-6) Alexa Fluor® 594 | sc-518157 AF594 | 200 µg/ml | $357.00 | |||
Angiopoietin 1/Ang-1/ANGPT1 Antibody (D-6) Alexa Fluor® 647 | sc-518157 AF647 | 200 µg/ml | $357.00 | |||
Angiopoietin 1/Ang-1/ANGPT1 Antibody (D-6) Alexa Fluor® 680 | sc-518157 AF680 | 200 µg/ml | $357.00 | |||
Angiopoietin 1/Ang-1/ANGPT1 Antibody (D-6) Alexa Fluor® 790 | sc-518157 AF790 | 200 µg/ml | $357.00 |