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Amylase Antibody (G-8): sc-166349

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Datasheets
  • Amylase Antibody (G-8) is a mouse monoclonal IgG1 κ, cited in 6 publications, provided at 200 µg/ml
  • specific for an epitope mapping between amino acids 385-417 near the C-terminus of Amylase of human origin
  • recommended for detection of precursor and mature amylase 1A (salivary), 2A and 2B (pancreatic) of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
  • See Amylase (G-10): sc-46657 for Amylase antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
  • m-IgG Fc BP-HRP is the preferred secondary detection reagent for Amylase Antibody (G-8) for WB and IHC(P) applications. This reagent is now offered in a bundle with Amylase Antibody (G-8) (see ordering information below).
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Amylase Antibody (G-8) is a mouse monoclonal IgG1 kappa light chain antibody designed to detect both precursor and mature forms of amylase 1A (salivary), as well as amylase 2A and 2B (pancreatic) across mouse, rat, and human species. Anti-amylase antibody (G-8) targets an epitope mapped to amino acids 385-417 near the C-terminus of human amylase, also known as alpha-amylase, ensuring precise recognition and binding. Amylase proteins play a critical role in carbohydrate metabolism by catalyzing the breakdown of starches into simpler sugars, which is essential for proper digestive function and energy production. Located primarily in the salivary glands and pancreas, amylase is secreted into the digestive tract where it facilitates efficient digestion of dietary starches. Understanding the cellular distribution and regulation of amylase is vital for researching digestive disorders, pancreatic health, and certain tumor pathologies, making amylase (G-8) antibody an invaluable tool for scientists in these areas. Amylase monoclonal antibody (G-8) performs reliably in a variety of applications, including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). Available in both non-conjugated form and multiple conjugated variants, such as horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and various Alexa Fluor® conjugates, amylase (G-8) monoclonal antibody supports advanced research into metabolic pathways, enzyme functions, and disease mechanisms related to amylase activity.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

Amylase Antibody (G-8)

sc-166349
200 µg/ml
$322.00

Amylase Antibody (G-8): m-IgG Fc BP-HRP Bundle

sc-540086
200 µg Ab; 10 µg BP
$361.00

Amylase (G-8) Neutralizing Peptide

sc-166349 P
100 µg/0.5 ml
$69.00

What application is the blocking peptide sc-166349 P appropriate for?

Asked by: Germaine
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Works great for mouse human Amylase transfectedWorks great for mouse human Amylase transfected lysates. -SCBT QC
Date published: 2015-06-11
Rated 5 out of 5 by from WB: strongWB: strong, clean signal in rat and human pancreas tissue extract. -SCBT QC
Date published: 2015-05-17
Rated 5 out of 5 by from Positive results in western blot analysisPositive results in western blot analysis of Amylase expression in human saliva. -SCBT QC
Date published: 2015-05-01
Rated 5 out of 5 by from Produced excellent immunoperoxidase cytoplasmicProduced excellent immunoperoxidase cytoplasmic staining in formalin fixed, paraffin-embedded human pancreas tissue. -SCBT QC
Date published: 2015-04-24
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Amylase Antibody (G-8) is rated 5.0 out of 5 by 4.
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