AMPK alpha 1 Antibody (H-4): sc-398861

AMPKα1 Antibody (H-4) is a mouse monoclonal IgG2b kappa light chain antibody that detects AMPK alpha 1 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). AMPKα1 (H-4) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. The 5′-AMP-activated protein kinase (AMPK) is a crucial heterotrimeric complex composed of a catalytic α subunit and regulatory β and γ subunits, playing a vital role in cellular energy homeostasis. AMPK is primarily located in the cytoplasm and becomes activated in response to cellular stressors that lead to ATP depletion, such as hypoxia or nutrient deprivation. This cytoplasmic location enables rapid sensing of energy status and initiates metabolic adaptations, including anabolic process inhibition and catabolic pathway activation to restore energy balance. AMPK activation leads to phosphorylation of key regulatory enzymes, such as hydroxy-methylglutaryl-CoA reductase and acetyl-CoA carboxylase, which are essential for sterol and fatty acid synthesis, respectively. The human AMPKα1 gene encodes a 548 amino acid protein, while AMPKα2 gene encodes a 552 amino acid protein, with both isoforms contributing to metabolic process regulation in response to energy fluctuations.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

AMPK alpha 1 Antibody (H-4) References:

1. Characterization of AMP-activated protein kinase gamma-subunit isoforms and their role in AMP binding.  |  Cheung, PC., et al. 2000. Biochem J. 346 Pt 3: 659-69. PMID: 10698692
2. Mammalian AMP-activated protein kinase subfamily.  |  Stapleton, D., et al. 1996. J Biol Chem. 271: 611-4. PMID: 8557660
3. The AMP-activated protein kinase--fuel gauge of the mammalian cell?  |  Hardie, DG. and Carling, D. 1997. Eur J Biochem. 246: 259-73. PMID: 9208914
4. AMP-activated protein kinase isoenzyme family: subunit structure and chromosomal location.  |  Stapleton, D., et al. 1997. FEBS Lett. 409: 452-6. PMID: 9224708
5. Identification of a novel AMP-activated protein kinase beta subunit isoform that is highly expressed in skeletal muscle.  |  Thornton, C., et al. 1998. J Biol Chem. 273: 12443-50. PMID: 9575201

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-AMPK alpha 1 Antibody (H-4) has 45 citations in a variety of scientific publications.

Primary antibodies like Anti-AMPK alpha 1 Antibody (H-4) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

AMPK alpha 1 Antibody (H-4) Specifications

Alternate Product Name: AMPKα1

Product Clone Name: H-4

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 509-533

Epitope Target: AMPKα1

Epitope Species: human

Product Reactivity/Detection: AMPKα1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: bovine

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 45 citations in various scientific publications

Available Conjugates/Forms for AMPK alpha 1 Antibody (H-4): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin

The preferred AMPK alpha 1/2 antibody is AMPK alpha 1/2 Antibody (D-6): sc-74461.