Amphiphysin I Antibody (A-7): sc-376435

Amphiphysin I Antibody (A-7) is a mouse monoclonal IgG1 antibody that detects Amphiphysin I in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Amphiphysin I is a crucial brain-enriched protein that plays a significant role in synaptic vesicle recycling, a process vital for neurotransmission. Anti-Amphiphysin I antibody (A-7) functions as a dimer and features a unique structure that includes a membrane-bending BAR domain, a central Clathrin and adaptor binding domain, and a C-terminal SH3 domain. The interaction between Amphiphysin I and the GTPase Dynamin, mediated by the SH3 domain, is essential for the proper binding of Dynamin to Clathrin-coated pits, facilitating vesicle budding and release at synapses. This mechanism is not only critical for neuronal communication but also highlights the importance of Amphiphysin I in maintaining synaptic integrity and plasticity. Additionally, a splice variant of Amphiphysin II, which lacks Clathrin or adaptor interactions, is predominantly expressed in muscle tissue and contributes to the formation and stabilization of the T tubule network, further underscoring the diverse functional roles of Amphiphysin proteins across different tissues. Amphiphysin I monoclonal antibody (A-7) is an invaluable tool for researchers studying synaptic dynamics and membrane trafficking in various biological contexts.

The preferred Amphiphysin I antibody is Amphiphysin I Antibody (G-4): sc-376402.