Amphiphysin I Antibody (G-4): sc-376402

Amphiphysin I Antibody (G-4) is a mouse monoclonal IgG2b antibody that detects Amphiphysin I of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). anti-Amphiphysin I antibody (G-4) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Amphiphysin I is a crucial brain-enriched protein that plays a significant role in synaptic vesicle recycling, a process vital for neurotransmission. Amphiphysin I′s structure includes a membrane-bending BAR domain, a central Clathrin and adaptor binding domain, and a C-terminal SH3 domain, which collectively facilitate function in the formation and stabilization of membrane structures. In the brain, Amphiphysin I and II form heterodimers that interact with the Clathrin-associated GTPase Dynamin through their SH3 domains, a critical step that precedes the binding of Dynamin to Clathrin-coated pits, leading to vesicle budding. This interaction is essential for maintaining synaptic function and ensuring efficient neurotransmitter release. Additionally, in other tissues, Amphiphysin I may contribute to various membrane bending and curvature stabilization events, highlighting importance beyond the nervous system. The mammalian Amphiphysins share a similar overall structure, with a splice variant of Amphiphysin II that is highly expressed in muscle tissue, where Amphiphysin II is involved in the formation and stabilization of the T tubule network, further underscoring the diverse roles of Amphiphysin proteins in cellular dynamics.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Amphiphysin I Antibody (G-4) References:

1. Amphiphysin, a novel protein associated with synaptic vesicles.  |  Lichte, B., et al. 1992. EMBO J. 11: 2521-30. PMID: 1628617
2. Primary structure of human amphiphysin, the dominant autoantigen of paraneoplastic stiff-man syndrome, and mapping of its gene (AMPH) to chromosome 7p13-p14.  |  Yamamoto, R., et al. 1995. Hum Mol Genet. 4: 265-8. PMID: 7757077
3. BIN1 is a novel MYC-interacting protein with features of a tumour suppressor.  |  Sakamuro, D., et al. 1996. Nat Genet. 14: 69-77. PMID: 8782822
4. A new member of the amphiphysin family connecting endocytosis and signal transduction pathways.  |  Leprince, C., et al. 1997. J Biol Chem. 272: 15101-5. PMID: 9182529
5. The putative tumor suppressor BIN1 is a short-lived nuclear phosphoprotein, the localization of which is altered in malignant cells.  |  Wechsler-Reya, R., et al. 1997. Cancer Res. 57: 3258-63. PMID: 9242458
6. Amphiphysin heterodimers: potential role in clathrin-mediated endocytosis.  |  Wigge, P., et al. 1997. Mol Biol Cell. 8: 2003-15. PMID: 9348539
7. Structural analysis of the human BIN1 gene. Evidence for tissue-specific transcriptional regulation and alternate RNA splicing.  |  Wechsler-Reya, R., et al. 1997. J Biol Chem. 272: 31453-8. PMID: 9395479

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Amphiphysin I Antibody (G-4) has 1 citations in a variety of scientific publications.

Primary antibodies like Anti-Amphiphysin I Antibody (G-4) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Amphiphysin I Antibody (G-4) Specifications

Alternate Product Name: Amphiphysin I

Product Clone Name: G-4

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b

Amino Acids: 2-20

Epitope Target: Amphiphysin

Epitope Species: human

Product Reactivity/Detection: Amphiphysin I

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2b in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 1 citations in various scientific publications

Available Conjugates/Forms for Amphiphysin I Antibody (G-4): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin