Amphiphysin I Antibody (13): sc-21711

Amphiphysin I Antibody (13) is a mouse monoclonal IgG1 antibody that detects Amphiphysin I in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), and immunofluorescence (IF). Amphiphysin I is a crucial brain-enriched protein that plays a significant role in synaptic vesicle recycling, a process vital for neurotransmission and communication between neurons. Anti-Amphiphysin I antibody (13) recognizes a protein that functions as a dimer and features a unique structure including a membrane-bending BAR domain, a central Clathrin and adaptor binding domain, and a C-terminal SH3 domain. The interaction of Amphiphysin I with the GTPase Dynamin via its SH3 domain is essential for proper binding of Dynamin to Clathrin-coated pits, facilitating the budding of synaptic vesicles. In addition to its role in the brain, Amphiphysin I is also implicated in membrane bending and curvature stabilization in various tissues, highlighting its importance in cellular dynamics. Notably, the mammalian Amphiphysins, including Amphiphysin I and its isoform Amphiphysin II, share a similar overall structure, yet they exhibit distinct functional roles, particularly in muscle tissue where a splice variant of Amphiphysin II contributes to the formation and stabilization of the T tubule network.

The preferred Amphiphysin I antibody is Amphiphysin I Antibody (G-4): sc-376402.