Date published: 2025-12-25

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AMIGO1 Antibody (C-10): sc-374419

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Datasheets
  • AMIGO1 Antibody (C-10) is a mouse monoclonal IgG2a κ provided at 200 µg/ml
  • raised against amino acids 269-387 mapping within an internal region of AMIGO1 of human origin
  • AMIGO1 Antibody (C-10) is recommended for detection of AMIGO1 of mouse, rat, human origin by WB, IP, IF and ELISA
  • Anti-AMIGO1 Antibody (C-10) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
  • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
  • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
  • m-IgG Fc BP-HRP is the preferred secondary detection reagent for AMIGO1 Antibody (C-10) for WB applications. This reagent is now offered in a bundle with AMIGO1 Antibody (C-10) (see ordering information below).

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    AMIGO1 Antibody (C-10) is a mouse monoclonal IgG2a kappa light chain antibody that detects AMIGO1 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-AMIGO1 antibody (C-10) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. The AMIGO family of proteins, which includes AMIGO-1, AMIGO-2, and AMIGO-3, consists of single-pass type I membrane proteins characterized by several leucine-rich repeats, an immunoglobulin domain, and a transmembrane domain. These proteins are predominantly located on the fiber tracts of neuronal tissues, where AMIGO proteins play a critical role in the formation and maintenance of neural connections. AMIGO-1, also known as Alivin-2, promotes the growth and fasciculation of neurites, which is vital for proper neural development and myelination of axons. AMIGO-1 can interact with other members of the AMIGO family, facilitating complex formation that enhances functional roles in cell signaling and communication. AMIGO proteins engage in homophilic and heterophilic interactions, highlighting their significance in neuronal development and nervous system integrity.

    For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

    Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA

    LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences

    Ordering Information

    Product NameCatalog #UNITPriceQtyFAVORITES

    AMIGO1 Antibody (C-10)

    sc-374419
    200 µg/ml
    $316.00

    AMIGO1 Antibody (C-10): m-IgG Fc BP-HRP Bundle

    sc-525961
    200 µg Ab; 10 µg BP
    $354.00

    AMIGO1 Antibody (C-10) AC

    sc-374419 AC
    500 µg/ml, 25% agarose
    $416.00

    AMIGO1 Antibody (C-10) HRP

    sc-374419 HRP
    200 µg/ml
    $316.00

    AMIGO1 Antibody (C-10) FITC

    sc-374419 FITC
    200 µg/ml
    $330.00

    AMIGO1 Antibody (C-10) PE

    sc-374419 PE
    200 µg/ml
    $343.00

    AMIGO1 Antibody (C-10) Alexa Fluor® 488

    sc-374419 AF488
    200 µg/ml
    $357.00

    AMIGO1 Antibody (C-10) Alexa Fluor® 546

    sc-374419 AF546
    200 µg/ml
    $357.00

    AMIGO1 Antibody (C-10) Alexa Fluor® 594

    sc-374419 AF594
    200 µg/ml
    $357.00

    AMIGO1 Antibody (C-10) Alexa Fluor® 647

    sc-374419 AF647
    200 µg/ml
    $357.00

    AMIGO1 Antibody (C-10) Alexa Fluor® 680

    sc-374419 AF680
    200 µg/ml
    $357.00

    AMIGO1 Antibody (C-10) Alexa Fluor® 790

    sc-374419 AF790
    200 µg/ml
    $357.00

    What is the recommended starting dilution for Western blot for AMIGO1 (C-10): sc-374419 monoclonal antibody?

    Asked by: jenniferc
    Thank you for your question. The recommended starting dilution for Western blot is 1:100 with a dilution range of 1:100-1:1000. This information, along with other technical specifications, can be found in the “datasheet” link at the top of the product page, for which I have provided the link: https://datasheets.scbt.com/sc-374419.pdf If you have any further questions or concerns, please contact our Technical Service department by calling 800-457-3801 option 2, emailing scbt@scbt.com, or using the Live Chat function on our website.
    Answered by: Technical Support
    Date published: 2017-03-01
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    Rated 5 out of 5 by from Nice Western blot data of AMIGO1 expression in nonNice Western blot data of AMIGO1 expression in non-transfected and human AMIGO1 transfected 293T whole cell lysates. -SCBT QC
    Date published: 2014-03-04
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    AMIGO1 Antibody (C-10) is rated 5.0 out of 5 by 1.
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