AMBRA1 Antibody (G-6): sc-398204
- AMBRA1 Antibody (G-6) is a mouse monoclonal IgG2a κ AMBRA1 antibody, cited in 18 publications, provided at 200 µg/ml
- raised against amino acids 999-1298 mapping at the C-terminus of AMBRA1 of human origin
- AMBRA1 Antibody (G-6) is recommended for detection of AMBRA1 of human origin by WB, IP, IF and ELISA
- Anti-AMBRA1 Antibody (G-6) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG2a BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for AMBRA1 Antibody (G-6) for WB applications. These reagents are now offered in bundles with AMBRA1 Antibody (G-6) (see ordering information below).
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SEE ALSO...
AMBRA1 Antibody (G-6) is a mouse monoclonal IgG2a kappa light chain antibody that detects AMBRA1 of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). AMBRA1 Antibody (G-6) is available in non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. AMBRA1, also known as Activating molecule in BECN1-regulated autophagy protein 1, WDR94, or KIAA1736, is a 1,298 amino acid protein characterized by three WD repeats, which are essential for function. These WD repeats form a tertiary propeller structure crucial for AMBRA1′s role in various cellular processes, including autophagy regulation, cell proliferation, and survival during neuronal development. AMBRA1 localizes to cytoplasmic vesicles, where AMBRA1 plays a significant role in controlling protein turnover and facilitating nervous system development. AMBRA1′s importance in autophagy highlights potential as a therapeutic target in neurodegenerative diseases, where autophagy dysregulation is often observed. Multiple AMBRA1 isoforms arise from alternative splicing events, potentially contributing to diverse functional roles in cellular homeostasis and stress responses.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
AMBRA1 Antibody (G-6) References:
- Prediction of the coding sequences of unidentified human genes. XIX. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro. | Nagase, T., et al. 2000. DNA Res. 7: 347-55. PMID: 11214970
- WD-repeat proteins: structure characteristics, biological function, and their involvement in human diseases. | Li, D. and Roberts, R. 2001. Cell Mol Life Sci. 58: 2085-97. PMID: 11814058
- Ambra1 regulates autophagy and development of the nervous system. | Fimia, GM., et al. 2007. Nature. 447: 1121-5. PMID: 17589504
- Autophagy: a new regulator of development. | Le Bot, N. 2007. Nat Cell Biol. 9: 741. PMID: 17603510
- A novel role for autophagy in neurodevelopment. | Cecconi, F., et al. 2007. Autophagy. 3: 506-8. PMID: 17622796
- Bcl-2 family members: dual regulators of apoptosis and autophagy. | Levine, B., et al. 2008. Autophagy. 4: 600-6. PMID: 18497563
- AMBRA1 regulates cyclin D to guard S-phase entry and genomic integrity. | Maiani, E., et al. 2021. Nature. 592: 799-803. PMID: 33854232
- The AMBRA1 E3 ligase adaptor regulates the stability of cyclin D. | Chaikovsky, AC., et al. 2021. Nature. 592: 794-798. PMID: 33854239
- AMBRA1 regulates mitophagy by interacting with ATAD3A and promoting PINK1 stability. | Di Rienzo, M., et al. 2022. Autophagy. 18: 1752-1762. PMID: 34798798
- AMBRA1 and its role as a target for anticancer therapy. | Li, X., et al. 2022. Front Oncol. 12: 946086. PMID: 36237336
- AMBRA1 phosphorylation by CDK1 and PLK1 regulates mitotic spindle orientation. | Faienza, F., et al. 2023. Cell Mol Life Sci. 80: 251. PMID: 37584777
- Role of AMBRA1 in mitophagy regulation: emerging evidence in aging-related diseases. | Di Rienzo, M., et al. 2024. Autophagy. 20: 2602-2615. PMID: 39113560
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
AMBRA1 Antibody (G-6) | sc-398204 | 200 µg/ml | $316.00 | |||
AMBRA1 Antibody (G-6): m-IgG Fc BP-HRP Bundle | sc-530718 | 200 µg Ab; 10 µg BP | $354.00 | |||
AMBRA1 Antibody (G-6): m-IgGκ BP-HRP Bundle | sc-524249 | 200 µg Ab, 40 µg BP | $354.00 | |||
AMBRA1 Antibody (G-6): m-IgG2a BP-HRP Bundle | sc-547570 | 200 µg Ab; 10 µg BP | $354.00 | |||
AMBRA1 Antibody (G-6) AC | sc-398204 AC | 500 µg/ml, 25% agarose | $416.00 | |||
AMBRA1 Antibody (G-6) HRP | sc-398204 HRP | 200 µg/ml | $316.00 | |||
AMBRA1 Antibody (G-6) FITC | sc-398204 FITC | 200 µg/ml | $330.00 | |||
AMBRA1 Antibody (G-6) PE | sc-398204 PE | 200 µg/ml | $343.00 | |||
AMBRA1 Antibody (G-6) Alexa Fluor® 488 | sc-398204 AF488 | 200 µg/ml | $357.00 | |||
AMBRA1 Antibody (G-6) Alexa Fluor® 546 | sc-398204 AF546 | 200 µg/ml | $357.00 | |||
AMBRA1 Antibody (G-6) Alexa Fluor® 594 | sc-398204 AF594 | 200 µg/ml | $357.00 | |||
AMBRA1 Antibody (G-6) Alexa Fluor® 647 | sc-398204 AF647 | 200 µg/ml | $357.00 | |||
AMBRA1 Antibody (G-6) Alexa Fluor® 680 | sc-398204 AF680 | 200 µg/ml | $357.00 | |||
AMBRA1 Antibody (G-6) Alexa Fluor® 790 | sc-398204 AF790 | 200 µg/ml | $357.00 |