ALX4 Antibody (KAB4): sc-33643
- ALX4 Antibody (KAB4) is a mouse monoclonal IgG1 κ ALX4 antibody, cited in 3 publications, provided at 200 µg/ml
- raised against purified recombinant full length ALX4 of mouse origin
- ALX4 Antibody (KAB4) is recommended for detection of ALX4 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
- Anti-ALX4 Antibody (KAB4) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for ALX4 Antibody (KAB4) for WB and IHC(P) applications. These reagents are now offered in bundles with ALX4 Antibody (KAB4) (see ordering information below).
QUICK LINKS
ALX4 Antibody (KAB4) is a mouse monoclonal IgG1 kappa light chain antibody that detects ALX4 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). ALX4 (KAB4) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. ALX4, also known as aristaless-like homeobox 4, functions as a DNA-binding transcription factor that plays a crucial role in the development of the skull and limbs, making ALX4 essential for proper skeletal formation. This nuclear protein is predominantly expressed in bone, and ALX4 defects are linked to parietal foramina 2 (PFM2), an autosomal dominant condition characterized by abnormal openings in the parietal bones due to insufficient ossification. PFM2 can also manifest as a feature of Potocki-Shaffer syndrome. Notably, bone morphogenetic proteins (BMP) have been shown to induce the expression of both Msx2 and ALX4 in calvarial mesenchyme, highlighting ALX4′s involvement in bone development pathways. Northern blot analysis has confirmed that ALX4 expression is restricted to bone tissue in both human and mouse models, underscoring ALX4′s significance in skeletal biology and potential implications in developmental disorders.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
ALX4 Antibody (KAB4) References:
- Haploinsufficiency of the human homeobox gene ALX4 causes skull ossification defects. | Mavrogiannis, LA., et al. 2001. Nat Genet. 27: 17-8. PMID: 11137991
- Severe nasal clefting and abnormal embryonic apoptosis in Alx3/Alx4 double mutant mice. | Beverdam, A., et al. 2001. Development. 128: 3975-86. PMID: 11641221
- Alx4 binding to LEF-1 regulates N-CAM promoter activity. | Boras, K. and Hamel, PA. 2002. J Biol Chem. 277: 1120-7. PMID: 11696550
- Survey for paired-like homeodomain gene expression in the hypothalamus: restricted expression patterns of Rx, Alx4 and goosecoid. | Asbreuk, CH., et al. 2002. Neuroscience. 114: 883-9. PMID: 12379244
- Progression of calvarial bone development requires Foxc1 regulation of Msx2 and Alx4. | Rice, R., et al. 2003. Dev Biol. 262: 75-87. PMID: 14512019
- Alx4 and Msx2 play phenotypically similar and additive roles in skull vault differentiation. | Antonopoulou, I., et al. 2004. J Anat. 204: 487-99. PMID: 15198690
- Physical and genetic interactions between Alx4 and Cart1. | Qu, S., et al. 1999. Development. 126: 359-69. PMID: 9847249
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ALX4 Antibody (KAB4) | sc-33643 | 200 µg/ml | $322.00 | |||
ALX4 Antibody (KAB4): m-IgG Fc BP-HRP Bundle | sc-528479 | 200 µg Ab; 10 µg BP | $361.00 | |||
ALX4 Antibody (KAB4): m-IgGκ BP-HRP Bundle | sc-520869 | 200 µg Ab, 40 µg BP | $361.00 | |||
ALX4 Antibody (KAB4): m-IgG1 BP-HRP Bundle | sc-542957 | 200 µg Ab; 20 µg BP | $361.00 | |||
ALX4 Antibody (KAB4) AC | sc-33643 AC | 500 µg/ml, 25% agarose | $424.00 | |||
ALX4 Antibody (KAB4) HRP | sc-33643 HRP | 200 µg/ml | $322.00 | |||
ALX4 Antibody (KAB4) FITC | sc-33643 FITC | 200 µg/ml | $336.00 | |||
ALX4 Antibody (KAB4) PE | sc-33643 PE | 200 µg/ml | $349.00 | |||
ALX4 Antibody (KAB4) Alexa Fluor® 488 | sc-33643 AF488 | 200 µg/ml | $364.00 | |||
ALX4 Antibody (KAB4) Alexa Fluor® 546 | sc-33643 AF546 | 200 µg/ml | $364.00 | |||
ALX4 Antibody (KAB4) Alexa Fluor® 594 | sc-33643 AF594 | 200 µg/ml | $364.00 | |||
ALX4 Antibody (KAB4) Alexa Fluor® 647 | sc-33643 AF647 | 200 µg/ml | $364.00 | |||
ALX4 Antibody (KAB4) Alexa Fluor® 680 | sc-33643 AF680 | 200 µg/ml | $364.00 | |||
ALX4 Antibody (KAB4) Alexa Fluor® 790 | sc-33643 AF790 | 200 µg/ml | $364.00 |