ALX1 Antibody (96K): sc-130416

ALX1 Antibody (96K) is a mouse monoclonal IgG2a kappa light chain antibody that detects ALX1 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), and immunofluorescence (IF). Anti-ALX1 antibody (96K) is available as non-conjugated format. ALX1, also known as CART-1 (cartilage homeoprotein-1), is a 326 amino acid transcription factor playing a crucial role in cervix and cartilage development. ALX1 (96K) antibody recognizes a protein characterized by a homeobox domain, essential for regulating gene expression, and an OAR domain that may facilitate DNA binding or protein-protein interactions, influencing transactivation processes. First identified in a rat chondrosarcoma tumor cell line, ALX1 acts as a transcriptional repressor for prolactin in vivo, highlighting importance in hormonal regulation. Studies have shown homozygous ALX1 deficient mice exhibit severe developmental defects, including acrania and meroanencephaly, underscoring a critical role in neural tube development. ALX1 interacts with ALX4, suggesting collaborative function in limb development and craniofacial bone formation, making ALX1 a significant target for research in developmental biology and related fields.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

ALX1 Antibody (96K) References:

1. The OAR/aristaless domain of the homeodomain protein Cart1 has an attenuating role in vivo.  |  Brouwer, A., et al. 2003. Mech Dev. 120: 241-52. PMID: 12559496
2. P300/CBP acts as a coactivator to cartilage homeoprotein-1 (Cart1), paired-like homeoprotein, through acetylation of the conserved lysine residue adjacent to the homeodomain.  |  Iioka, T., et al. 2003. J Bone Miner Res. 18: 1419-29. PMID: 12929931
3. Cartilage homeoprotein 1, a homeoprotein selectively expressed in chondrocytes.  |  Zhao, GQ., et al. 1993. Proc Natl Acad Sci U S A. 90: 8633-7. PMID: 7690966
4. Prenatal folic acid treatment suppresses acrania and meroanencephaly in mice mutant for the Cart1 homeobox gene.  |  Zhao, Q., et al. 1996. Nat Genet. 13: 275-83. PMID: 8673125
5. Elucidation of homeoprotein Cart-1 function during in vitro chondrogenesis of C3H10T1/2 micromass cultures.  |  Atkinson, BL., et al. 1996. Ann N Y Acad Sci. 785: 206-8. PMID: 8702130
6. Human Cart-1: structural organization, chromosomal localization, and functional analysis of a cartilage-specific homeodomain cDNA.  |  Gordon, DF., et al. 1996. DNA Cell Biol. 15: 531-41. PMID: 8756334
7. Human CART1, a paired-class homeodomain protein, activates transcription through palindromic binding sites.  |  Cai, RL. 1998. Biochem Biophys Res Commun. 250: 305-11. PMID: 9753625
8. Physical and genetic interactions between Alx4 and Cart1.  |  Qu, S., et al. 1999. Development. 126: 359-69. PMID: 9847249

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-ALX1 Antibody (96K) has 1 citations in a variety of scientific publications.

Primary antibodies like Anti-ALX1 Antibody (96K) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

ALX1 Antibody (96K) Specifications

Alternate Product Name: ALX1

Product Clone Name: 96K

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 198-307

Epitope Target: ALX1

Epitope Species: human

Product Reactivity/Detection: ALX1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF

Vialing Information: Each vial contains 100 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 1 citations in various scientific publications

Available Conjugates/Forms for ALX1 Antibody (96K): N/A