αPAK Antibody (A-6): sc-166887

αPAK Antibody (A-6) is a mouse monoclonal IgG1 kappa light chain antibody that detects αPAK in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). αPAK (A-6) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. αPAK, a member of the p21-activated kinase (PAK) family, plays a crucial role in cellular signaling pathways by acting as a downstream effector of the small GTPases Rac1 and Cdc42, which are pivotal in regulating cytoskeletal dynamics, cell motility, and growth. αPAK interaction with these GTPases is essential for activation, leading to autophosphorylation at specific serine and threonine residues, including Ser 19, Ser 141, and Thr 402. Notably, phosphorylation at Ser 141 and Thr 402 directly correlates with γPAK activation, one of the PAK family isoforms. Upon activation, PAK isoforms dissociate from GTPase partners to phosphorylate downstream substrates, such as Mek kinase, which is involved in the c-Jun N-terminal kinase (JNK) signaling pathway. This intricate regulation of signaling pathways underscores αPAK importance in various cellular processes, making anti-αPAK antibody (A-6) a valuable tool for researchers studying these critical mechanisms in cell biology.

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αPAK Antibody (A-6) References:

1. p21-activated protein kinase gamma-PAK is activated by ionizing radiation and other DNA-damaging agents. Similarities and differences to alpha-PAK.  |  Roig, J. and Traugh, JA. 1999. J Biol Chem. 274: 31119-22. PMID: 10531298
2. Molecular cloning of the gene for the human placental GTP-binding protein Gp (G25K): identification of this GTP-binding protein as the human homolog of the yeast cell-division-cycle protein CDC42.  |  Shinjo, K., et al. 1990. Proc Natl Acad Sci U S A. 87: 9853-7. PMID: 2124704
3. rac, a novel ras-related family of proteins that are botulinum toxin substrates.  |  Didsbury, J., et al. 1989. J Biol Chem. 264: 16378-82. PMID: 2674130
4. The small GTP-binding proteins Rac1 and Cdc42 regulate the activity of the JNK/SAPK signaling pathway.  |  Coso, OA., et al. 1995. Cell. 81: 1137-46. PMID: 7600581
5. A novel serine kinase activated by rac1/CDC42Hs-dependent autophosphorylation is related to PAK65 and STE20.  |  Martin, GA., et al. 1995. EMBO J. 14: 1970-8. PMID: 7744004
6. Differential activation of ERK and JNK mitogen-activated protein kinases by Raf-1 and MEKK.  |  Minden, A., et al. 1994. Science. 266: 1719-23. PMID: 7992057
7. Activation of stress-activated protein kinase by MEKK1 phosphorylation of its activator SEK1.  |  Yan, M., et al. Nature. 372: 798-800. PMID: 7997270
8. A brain serine/threonine protein kinase activated by Cdc42 and Rac1.  |  Manser, E., et al. 1994. Nature. 367: 40-6. PMID: 8107774
9. Proteins regulating Ras and its relatives.  |  Boguski, MS. and McCormick, F. 1993. Nature. 366: 643-54. PMID: 8259209
10. Expression of constitutively active alpha-PAK reveals effects of the kinase on actin and focal complexes.  |  Manser, E., et al. 1997. Mol Cell Biol. 17: 1129-43. PMID: 9032240
11. Activation of p21-activated protein kinase alpha (alpha PAK) by hyperosmotic shock in neonatal ventricular myocytes.  |  Clerk, A. and Sugden, PH. 1997. FEBS Lett. 403: 23-5. PMID: 9038353
12. A conserved negative regulatory region in alphaPAK: inhibition of PAK kinases reveals their morphological roles downstream of Cdc42 and Rac1.  |  Zhao, ZS., et al. 1998. Mol Cell Biol. 18: 2153-63. PMID: 9528787

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-αPAK Antibody (A-6) has 14 citations in a variety of scientific publications.

Primary antibodies like Anti-αPAK Antibody (A-6) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

αPAK Antibody (A-6) Specifications

Alternate Product Name: αPAK

Product Clone Name: A-6

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 246-470

Epitope Target: αPAK

Epitope Species: human

Product Reactivity/Detection: αPAK, βPAK and γPAK

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: equine and bovine

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 14 citations in various scientific publications

Available Conjugates/Forms for αPAK Antibody (A-6): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred p-αPAK antibody is p-αPAK Antibody (17.Thr 423): sc-135755.