p-αPAK Antibody (17.Thr 423): sc-135755

p-αPAK Antibody (17.Thr 423) is a mouse monoclonal IgG2b kappa light chain antibody that detects the Thr 423 phosphorylated αPAK protein of mouse, rat, and human origin by western blotting (WB) and enzyme-linked immunosorbent assay (ELISA). anti-p-αPAK antibody (17.Thr 423) is available in both conjugated and non-conjugated forms, providing flexibility for various experimental needs. The p21-activated kinases (PAK) play a crucial role in cellular signaling, linking Rho GTPases to cytoskeletal reorganization and nuclear signaling, which is vital for processes such as cell migration, proliferation, and survival. Among the three common isoforms—αPAK p68, βPAK p65, and γPAK p62—αPAK is particularly significant due to involvement in the activation of downstream signaling pathways. Phosphorylation of αPAK at Thr 423, catalyzed by Cdc42, is essential for activation, allowing dissociation from the Rac/Cdc42 complex and interaction with downstream substrates like MEK kinase, which is a key player in the JNK signaling pathway. Understanding the dynamics of αPAK phosphorylation and interactions is critical for elucidating the mechanisms of various cellular responses and could provide insights into therapeutic targets for diseases where these pathways are dysregulated.

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p-αPAK Antibody (17.Thr 423) References:

1. Identification of a central phosphorylation site in p21-activated kinase regulating autoinhibition and kinase activity.  |  Zenke, FT., et al. 1999. J Biol Chem. 274: 32565-73. PMID: 10551809
2. Molecular cloning of the gene for the human placental GTP-binding protein Gp (G25K): identification of this GTP-binding protein as the human homolog of the yeast cell-division-cycle protein CDC42.  |  Shinjo, K., et al. 1990. Proc Natl Acad Sci U S A. 87: 9853-7. PMID: 2124704
3. rac, a novel ras-related family of proteins that are botulinum toxin substrates.  |  Didsbury, J., et al. 1989. J Biol Chem. 264: 16378-82. PMID: 2674130
4. The small GTP-binding proteins Rac1 and Cdc42 regulate the activity of the JNK/SAPK signaling pathway.  |  Coso, OA., et al. 1995. Cell. 81: 1137-46. PMID: 7600581
5. A novel serine kinase activated by rac1/CDC42Hs-dependent autophosphorylation is related to PAK65 and STE20.  |  Martin, GA., et al. 1995. EMBO J. 14: 1970-8. PMID: 7744004
6. Differential activation of ERK and JNK mitogen-activated protein kinases by Raf-1 and MEKK.  |  Minden, A., et al. 1994. Science. 266: 1719-23. PMID: 7992057
7. Activation of stress-activated protein kinase by MEKK1 phosphorylation of its activator SEK1.  |  Yan, M., et al. Nature. 372: 798-800. PMID: 7997270
8. A brain serine/threonine protein kinase activated by Cdc42 and Rac1.  |  Manser, E., et al. 1994. Nature. 367: 40-6. PMID: 8107774
9. Proteins regulating Ras and its relatives.  |  Boguski, MS. and McCormick, F. 1993. Nature. 366: 643-54. PMID: 8259209
10. Expression of constitutively active alpha-PAK reveals effects of the kinase on actin and focal complexes.  |  Manser, E., et al. 1997. Mol Cell Biol. 17: 1129-43. PMID: 9032240

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-p-αPAK Antibody (17.Thr 423) has 4 citations in a variety of scientific publications.

Primary antibodies like Anti-p-αPAK Antibody (17.Thr 423) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

p-αPAK Antibody (17.Thr 423) Specifications

Alternate Product Name: αPAK

Product Clone Name: 17.Thr 423

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: Thr 423

Epitope Target: αPAK

Epitope Species: human

Product Reactivity/Detection: Thr 423 phosphorylated αPAK of mouse and human origin, and corresponding rat homolog

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, ELISA

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 4 citations in various scientific publications

Available Conjugates/Forms for p-αPAK Antibody (17.Thr 423): AC: Agarose, HRP: Horseradish Peroxidase

The preferred αPAK antibody is αPAK Antibody (A-6): sc-166887.