αA-crystallin Antibody (B-2): sc-28306

αA-crystallin Antibody (B-2) is a mouse monoclonal IgG2a kappa light chain antibody that detects αA-crystallin of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). αA-crystallin monoclonal antibody (B-2) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. αA-crystallin plays a crucial role in maintaining the transparency and refractive index of the vertebrate eye lens, which is essential for proper vision. As a member of the small heat shock protein family, αA-crystallin functions as a molecular chaperone, helping to stabilize denatured proteins and prevent aggregation, although αA-crystallin does not facilitate their renaturation. αA-crystallin is primarily expressed in the lens, and mutations in the αA-crystallin gene can lead to autosomal dominant congenital cataracts, highlighting αA-crystallin′s importance in lens integrity and function. Additionally, αA-crystallin interacts with other crystallins and proteins involved in cellular stress responses, further underscoring αA-crystallin′s significance in maintaining cellular homeostasis and protecting against proteotoxicity.

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αA-crystallin Antibody (B-2) References:

1. Mutation R120G in alphaB-crystallin, which is linked to a desmin-related myopathy, results in an irregular structure and defective chaperone-like function.  |  Bova, MP., et al. 1999. Proc Natl Acad Sci U S A. 96: 6137-42. PMID: 10339554
2. alpha-crystallin prevents irreversible protein denaturation and acts cooperatively with other heat-shock proteins to renature the stabilized partially denatured protein in an ATP-dependent manner.  |  Wang, K. and Spector, A. 2000. Eur J Biochem. 267: 4705-12. PMID: 10903503
3. Lens crystallins and their microbial homologs: structure, stability, and function.  |  Jaenicke, R. and Slingsby, C. 2001. Crit Rev Biochem Mol Biol. 36: 435-99. PMID: 11724156
4. Alpha-crystallin-type heat shock proteins: socializing minichaperones in the context of a multichaperone network.  |  Narberhaus, F. 2002. Microbiol Mol Biol Rev. 66: 64-93; table of contents. PMID: 11875128
5. The small heat shock protein alpha B-crystallin is a novel inhibitor of TRAIL-induced apoptosis that suppresses the activation of caspase-3.  |  Kamradt, MC., et al. 2005. J Biol Chem. 280: 11059-66. PMID: 15653686
6. The Role of αA-Crystallin in Experimental Autoimmune Uveitis.  |  Wang, L., et al. 2015. Curr Mol Med. 15: 558-64. PMID: 26238368
7. Membrane insertion of αA-crystallin is oligomer-size dependent.  |  Tjondro, HC., et al. 2016. Biochem Biophys Res Commun. 473: 1-7. PMID: 26975472
8. Evidence for Paracrine Protective Role of Exogenous αA-Crystallin in Retinal Ganglion Cells.  |  Nath, M., et al. 2022. eNeuro. 9: PMID: 35168949
9. αA-Crystallin Mediated Neuroprotection in the Retinal Neurons Is Independent of Protein Kinase B.  |  Nath, M. and Fort, PE. 2022. Front Neurosci. 16: 912757. PMID: 35669493
10. Differential expression of B-crystallin and Hsp27 in skeletal muscle during continuous contractile activity. Relationship to myogenic regulatory factors.  |  Neufer, PD. and Benjamin, IJ. 1996. J Biol Chem. 271: 24089-95. PMID: 8798647
11. Autosomal dominant congenital cataract associated with a missense mutation in the human alpha crystallin gene CRYAA.  |  Litt, M., et al. 1998. Hum Mol Genet. 7: 471-4. PMID: 9467006
12. The small heat-shock protein, alphaB-crystallin, has a variable quaternary structure.  |  Haley, DA., et al. 1998. J Mol Biol. 277: 27-35. PMID: 9514758

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-αA-crystallin Antibody (B-2) has 19 citations in a variety of scientific publications.

Primary antibodies like Anti-αA-crystallin Antibody (B-2) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

αA-crystallin Antibody (B-2) Specifications

Alternate Product Name: αA-crystallin

Product Clone Name: B-2

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 1-173

Epitope Target: αA-crystallin

Epitope Species: human

Product Reactivity/Detection: αA-crystallin

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 19 citations in various scientific publications

Available Conjugates/Forms for αA-crystallin Antibody (B-2): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin