alpha Tubulin Antibody (B-5-1-2): sc-23948

α Tubulin Antibody (B-5-1-2) is a mouse monoclonal IgG1 kappa light chain antibody, raised against Sarkosyl-resistant ribbons from Strongylocentrotus purpuratus (sea urchin) sperm axonemes. anti-α Tubulin antibody (B-5-1-2) detects α Tubulin across a broad range of species, including human, mouse, rat, and others, in applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry with paraffin-embedded sections (IHCP). Tubulin, a crucial structural protein of the cytoskeleton, exists in multiple isoforms, α, β, γ, δ, and ε, that form the basis of microtubules, essential for processes such as cell division, intracellular transport, and maintaining cell shape. α Tubulin forms heterodimers with β Tubulin to assemble into microtubules, which provide scaffolding for cellular structures and facilitate proper alignment and segregation of chromosomes during mitosis. Post-translational modifications of α Tubulin, including acetylation, detyrosination, and phosphorylation, play significant roles in regulating microtubule stability and dynamics, influencing cellular processes like motility and signaling. α Tubulin monoclonal antibody (B-5-1-2) is recommended for researchers studying tubulin′s structural roles, post-translational modifications, cytoskeletal dynamics and cellular regulation. α Tubulin (B-5-1-2) antibody is available in both non-conjugated and conjugated formats, including horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), agarose, and multiple Alexa Fluor® conjugates, providing flexibility for various experimental setups.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

alpha Tubulin Antibody (B-5-1-2) References:

1. Presence of the betaII isotype of tubulin in the nuclei of cultured mesangial cells from rat kidney.  |  Walss, C., et al. 1999. Cell Motil Cytoskeleton. 42: 274-84. PMID: 10223634
2. Identification of betaIII- and betaIV-tubulin isotypes in cold-adapted microtubules from Atlantic cod (Gadus morhua): antibody mapping and cDNA sequencing.  |  Modig, C., et al. 1999. Cell Motil Cytoskeleton. 42: 315-30. PMID: 10223637
3. Delta-tubulin and epsilon-tubulin: two new human centrosomal tubulins reveal new aspects of centrosome structure and function.  |  Chang, P. and Stearns, T. 2000. Nat Cell Biol. 2: 30-5. PMID: 10620804
4. Tubulin immunoreactive neuronal intranuclear inclusions in the human brain.  |  Woulfe, J. and Munoz, D. 2000. Neuropathol Appl Neurobiol. 26: 161-71. PMID: 10840279
5. Alpha-, beta-, and gamma-tubulins: sequence comparisons and structural constraints.  |  Burns, RG. 1991. Cell Motil Cytoskeleton. 20: 181-9. PMID: 1773446
6. Gamma-tubulin is present in Drosophila melanogaster and Homo sapiens and is associated with the centrosome.  |  Zheng, Y., et al. 1991. Cell. 65: 817-23. PMID: 1904010
7. Phosphomimetic Mutation at Ser165 of α-Tubulin Promotes the Persistence of GTP Caps in Microtubules.  |  Maddula, V., et al. 2022. Biochemistry. 61: 1508-1516. PMID: 35799350
8. Molecular Docking of Cryptoconcatones to α-Tubulin and Related Pironetin Analogues.  |  Vergoten, G. and Bailly, C. 2023. Plants (Basel). 12: PMID: 36679009
9. Invited review: the role of nucleotide triphosphate in actin and tubulin assembly and function.  |  Weisenberg, RC. 1981. Cell Motil. 1: 485-97. PMID: 6756643
10. Multiple forms of tubulin: different gene products and covalent modifications.  |  Ludueña, RF. 1998. Int Rev Cytol. 178: 207-75. PMID: 9348671
11. Expression of amino- and carboxyl-terminal gamma- and alpha-tubulin mutants in cultured epithelial cells.  |  Leask, A. and Stearns, T. 1998. J Biol Chem. 273: 2661-8. PMID: 9446570
12. Formation and function of the Rbl2p-beta-tubulin complex.  |  Archer, JE., et al. 1998. Mol Cell Biol. 18: 1757-62. PMID: 9488492

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-alpha Tubulin Antibody (B-5-1-2) has 574 citations in a variety of scientific publications.

Primary antibodies like Anti-alpha Tubulin Antibody (B-5-1-2) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

alpha Tubulin Antibody (B-5-1-2) Specifications

Alternate Product Name: α Tubulin

Product Clone Name: B-5-1-2

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 149-448

Epitope Target: α Tubulin

Epitope Species: human

Product Reactivity/Detection: α Tubulin

Species Reactivity/Detection: multiple

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P)

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 574 citations in various scientific publications

Available Conjugates/Forms for alpha Tubulin Antibody (B-5-1-2): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred alpha Tubulin antibody is alpha Tubulin Antibody (TU-02): sc-8035.