alpha Tubulin Antibody (TU-02) recommended for detection of α Tubulin of mouse, rat, human and porcine origin by WB, IP, IF, IHC(P), FCM and ELISA

α Tubulin Antibody (TU-02): sc-8035

α Tubulin Antibody (TU-02) is rated 4.7 out of 5 by 73.
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Datasheets

    • α Tubulin Antibody (TU-02) is a mouse monoclonal IgM (kappa light chain) provided at 200 µg/ml
    • raised against amino acids 1-451 representing full length α Tubulin of porcine origin
    • Anti-alpha Tubulin Antibody (TU-02) is recommended for detection of α Tubulin of mouse, rat, human and porcine origin by WB, IP, IF, IHC(P), FCM and ELISA
    • Anti-alpha Tubulin Antibody (TU-02) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • available conjugated to agarose (sc-8035 AC), 500 µg/ml, 25% agarose, for IP; and to phycoerythrin (sc-8035 PE), 200 µg/ml, for IF, IHC(P) and FCM
    • also available conjugated to either TRITC, PerCP, PerCP-Cy5.5 or Alexa Fluor® 405 for IF, IHC(P) and FCM
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of α Tubulin (TU-02): sc-8035.

Hi, I have purchased this antibody a few weeks ago. Initially, the band took so long to appear at 55kDa. The second time I tried, another band appear at 45kDa which is more intense. The band at 55kDa took more than 3 hours to develop

Asked by: Siti Khadijah
Thank you for your message. Please get in touch with our technical service team directly so we can troubleshoot your experiment.
Answered by: Tech Support Europe
Date published: 2020-08-05

Liebes Santa Cruz Team, besteht die Möglichkeit ein Testsample oder kleineres Muster von α Tubulin Antikörper (TU-02): sc-8035 mit FITC oder ähnlichem konjugiert zu erhalten? In welcher Verdünnung wird der AK für die IF eingesetzt? Vielen dank

Asked by: AKKlein
Vielen Dank für Ihre Anfrage. Wir bieten nur unkonjugierte Primärantikörper als kostenlose Samples an. Sie können auch unsere empfohlenen sekundären Bindeproteine als Samples erhalten, wie z.B. m-IgGκ BP-FITC: sc-516140 oder m-IgGκ BP-CFL 488: sc-516176. Kontaktieren Sie gerne unseren technischen Service um die Lieferung entsprechender Samples zu arrangieren.
Answered by: Tech Support Europe
Date published: 2019-06-14

Has this antibody been used on mouse brain sections?

Asked by: Alex3
Thank you for your question. This antibody is fine for mouse tissue sections, so it should be fine for your mouse brain samples. If you have any further questions, please reach out to Technical support at [email protected], or via phone at 800-457-3801, ext 2 or via chat directly on our website, www.scbt.com.
Answered by: Tech Service
Date published: 2017-12-04

Can I use this mouse monoclonal antibody for immunohistochemical staining of mouse tissue?

Asked by: Cweed
Yes. We recommend using a directly conjugated mouse monoclonal primary antibody. SC-8035 is currently available conjugated to FITC, AF488, AF647.
Answered by: TechService7
Date published: 2016-12-14

How can this mouse monoclonal antibody be used for double or triple staining, if the other primary antibodies are also raised in mouse?

Asked by: Cweed
In this case, we recommend using a directly conjugated mouse monoclonal primary antibody. SC-8035 is currently available conjugated to AF488, AF647, and FITC.
Answered by: TechService7
Date published: 2016-12-14
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Rated 5 out of 5 by from 很好用 帮客户买的,客户评价很好用,条带清晰。没有杂带。会继续回购的。对课题很有帮助。希望多一点折扣。经费在燃烧
Date published: 2020-09-18
Rated 5 out of 5 by from Good antibody for western blotting a-Tubulin Antibody (TU-02) is good quality for western blotting (dilute factor: 1:5000)
Date published: 2020-05-25
Rated 5 out of 5 by from reliable and specific in IF and WB specific staining in IF (dilution 1:100), if the cells (HEp-2, RKO, LoVo) were fixed in 4% PFA at 37°C. Works very well in WB (dilution 1:1000 in BSA/TBS-T) and shows a clear and strong signal for HEp-2, RKO and LoVo whole cell lysates
Date published: 2020-01-09
Rated 5 out of 5 by from Useful very good in Western blot ,and band is strong.
Date published: 2019-10-16
Rated 5 out of 5 by from Excellent antibody Purchased a year ago and happy using it. Antibody showed perfect bands in western blotting.
Date published: 2019-08-08
Rated 5 out of 5 by from Ottimo anticorpo Utilizzato per western blot, specie human. L'anticorpo ha soddisfatto le esigenze sperimentali.
Date published: 2019-02-04
Rated 5 out of 5 by from single and strong band We tried the sample and it shows one single strong band. Thanks
Date published: 2018-03-23
Rated 5 out of 5 by from Great antibody! 1:500 diluted for wb of rat protein,the band is special and strong
Date published: 2018-03-21
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