α Enolase Antibody (L-27): sc-100812

α Enolase Antibody (L-27) is a mouse monoclonal IgG1 kappa light chain antibody that detects α Enolase in mouse, rat, and human samples through various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). α Enolase monoclonal antibody (L-27) is offered in a non-conjugated form, allowing for versatile experimental setups. α Enolase (L-27) antibody recognizes a protein that plays a crucial role in the glycolytic pathway, facilitating the conversion of 2-phosphoglycerate to phosphoenolpyruvate, which is a key step in energy production. This enzymatic function is vital for cellular metabolism, particularly in tissues with high energy demands, such as muscle and brain. Notably, α Enolase is not only involved in glycolysis but also has been implicated in various cellular processes, including differentiation and stress responses. α Enolase expression across a wide range of tissues underscores its importance in maintaining metabolic homeostasis, and expression levels can serve as a biomarker for certain pathological conditions. With an ability to form both homodimers and heterodimers with other enolase isoforms, α Enolase′s structural versatility further enhances its functional significance in cellular metabolism.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

α Enolase Antibody (L-27) References:

1. Alpha-enolase plasminogen receptor in myogenesis.  |  Lopez-Alemany, R., et al. 2005. Front Biosci. 10: 30-6. PMID: 15574344
2. Differential expression of the human alpha-enolase gene in oral epithelium and squamous cell carcinoma.  |  Ito, S., et al. 2007. Cancer Sci. 98: 499-505. PMID: 17284257
3. The kelch protein NS1-BP interacts with alpha-enolase/MBP-1 and is involved in c-Myc gene transcriptional control.  |  Perconti, G., et al. 2007. Biochim Biophys Acta. 1773: 1774-85. PMID: 17996313
4. Progress in the biological function of alpha-enolase.  |  Ji, H., et al. 2016. Anim Nutr. 2: 12-17. PMID: 29767008
5. Targeting Endothelial ENO1 (Alpha-Enolase) -PI3K-Akt-mTOR Axis Alleviates Hypoxic Pulmonary Hypertension.  |  Shi, Y., et al. 2023. Hypertension. 80: 1035-1047. PMID: 37075135
6. Keratin 17 covalently binds to alpha-enolase and exacerbates proliferation of keratinocytes in psoriasis.  |  Luo, Y., et al. 2023. Int J Biol Sci. 19: 3395-3411. PMID: 37497003
7. Synapse formation is related to the onset of neuron-specific enolase immunoreactivity in the avian auditory and vestibular systems.  |  Whitehead, MC., et al. 1982. Dev Neurosci. 5: 298-307. PMID: 7140580
8. DNA sequences encoding enolase are remarkably conserved from yeast to mammals.  |  Verma, M. and Dutta, SK. 1994. Life Sci. 55: 893-9. PMID: 7520111
9. Coexpression of alpha and gamma enolase genes in neurons of adult rat brain.  |  Keller, A., et al. 1994. J Neurosci Res. 38: 493-504. PMID: 7815469
10. A comparative study of the distribution of alpha- and gamma-enolase subunits in cultured rat neural cells and fibroblasts.  |  Deloulme, JC., et al. 1997. Int J Dev Neurosci. 15: 183-94. PMID: 9178037
11. Expression of two neuronal markers, growth-associated protein 43 and neuron-specific enolase, in rat glial cells.  |  Sensenbrenner, M., et al. 1997. J Mol Med (Berl). 75: 653-63. PMID: 9351704
12. Mechanism of enolase: the crystal structure of asymmetric dimer enolase-2-phospho-D-glycerate/enolase-phosphoenolpyruvate at 2.0 A resolution.  |  Zhang, E., et al. 1997. Biochemistry. 36: 12526-34. PMID: 9376357

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-α Enolase Antibody (L-27) has 32 citations in a variety of scientific publications.

Primary antibodies like Anti-α Enolase Antibody (L-27) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

α Enolase Antibody (L-27) Specifications

Alternate Product Name: α Enolase

Product Clone Name: L-27

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 1-435

Epitope Target: α Enolase

Epitope Species: human

Product Reactivity/Detection: α Enolase

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 100 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 32 citations in various scientific publications

Available Conjugates/Forms for α Enolase Antibody (L-27): N/A