ALAS-H Antibody (F-5): sc-137093
- ALAS-H Antibody (F-5) is a mouse monoclonal IgG1 κ ALAS-H antibody, cited in 3 publications, provided at 200 µg/ml
- raised against amino acids 57-210 mapping near the N-terminus of ALAS-H of mouse origin
- ALAS-H Antibody (F-5) is recommended for detection of precursor and mature ALAS-H of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-ALAS-H Antibody (F-5) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for ALAS-H Antibody (F-5) for WB applications. These reagents are now offered in bundles with ALAS-H Antibody (F-5) (see ordering information below).
ALAS-H Antibody (F-5) is a mouse monoclonal IgG1 kappa light chain antibody that detects ALAS-H of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). ALAS-H (F-5) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. The 5-aminolevulinate synthase 1 (ALAS-H) protein plays a crucial role in the heme biosynthetic pathway, catalyzing the first step in heme production, which is essential for hemoglobin and various cytochromes formation. ALAS-H is predominantly located in mitochondria of most cells, where ALAS-H facilitates early heme synthesis, a vital component for oxygen transport and cellular respiration. Proper functioning of ALAS-H is critical, as disruptions in heme synthesis can lead to various hematological disorders, including anemia. ALAS-H operates alongside ALAS-E isoform, which is specifically involved in hematopoietic differentiation and iron metabolism. ALAS-H interaction with succinyl CoA is distinct from ALAS-E, highlighting unique regulatory mechanisms governing heme synthesis in different cellular contexts. Since the ALAS gene is located on the X chromosome, mutations can result in pyridoxine-refractory X-linked sideroblastic anemia, underscoring ALAS-H importance in maintaining normal erythropoiesis and overall cellular function.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
ALAS-H Antibody (F-5) References:
- Interaction between succinyl CoA synthetase and the heme-biosynthetic enzyme ALAS-E is disrupted in sideroblastic anemia. | Furuyama, K. and Sassa, S. 2000. J Clin Invest. 105: 757-64. PMID: 10727444
- Transcriptional regulation of the murine erythroid-specific 5-aminolevulinate synthase gene. | Kramer, MF., et al. 2000. Gene. 247: 153-66. PMID: 10773455
- Transient state kinetic investigation of 5-aminolevulinate synthase reaction mechanism. | Zhang, J. and Ferreira, GC. 2002. J Biol Chem. 277: 44660-9. PMID: 12191993
- Human erythroid 5-aminolevulinate synthase. Gene structure and species-specific differences in alternative RNA splicing. | Conboy, JG., et al. 1992. J Biol Chem. 267: 18753-8. PMID: 1527005
- Differential regulation of human ALAS1 mRNA and protein levels by heme and cobalt protoporphyrin. | Zheng, J., et al. 2008. Mol Cell Biochem. 319: 153-61. PMID: 18719978
- Peroxisome proliferator-activated receptor alpha controls hepatic heme biosynthesis through ALAS1. | Degenhardt, T., et al. 2009. J Mol Biol. 388: 225-38. PMID: 19289130
- Functional analysis of the 5' regulatory region of the 5-aminolevulinate synthase (ALAS1) gene in response to estrogen. | du Plessis, N., et al. 2009. Cell Mol Biol (Noisy-le-grand). 55: 20-30. PMID: 19656447
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ALAS-H Antibody (F-5) | sc-137093 | 200 µg/ml | $316.00 | |||
ALAS-H Antibody (F-5): m-IgG Fc BP-HRP Bundle | sc-528860 | 200 µg Ab; 10 µg BP | $354.00 | |||
ALAS-H Antibody (F-5): m-IgGκ BP-HRP Bundle | sc-521425 | 200 µg Ab, 40 µg BP | $354.00 | |||
ALAS-H Antibody (F-5): m-IgG1 BP-HRP Bundle | sc-543173 | 200 µg Ab; 20 µg BP | $354.00 | |||
ALAS-H Antibody (F-5) AC | sc-137093 AC | 500 µg/ml, 25% agarose | $416.00 | |||
ALAS-H Antibody (F-5) HRP | sc-137093 HRP | 200 µg/ml | $316.00 | |||
ALAS-H Antibody (F-5) FITC | sc-137093 FITC | 200 µg/ml | $330.00 | |||
ALAS-H Antibody (F-5) PE | sc-137093 PE | 200 µg/ml | $343.00 | |||
ALAS-H Antibody (F-5) Alexa Fluor® 488 | sc-137093 AF488 | 200 µg/ml | $357.00 | |||
ALAS-H Antibody (F-5) Alexa Fluor® 546 | sc-137093 AF546 | 200 µg/ml | $357.00 | |||
ALAS-H Antibody (F-5) Alexa Fluor® 594 | sc-137093 AF594 | 200 µg/ml | $357.00 | |||
ALAS-H Antibody (F-5) Alexa Fluor® 647 | sc-137093 AF647 | 200 µg/ml | $357.00 | |||
ALAS-H Antibody (F-5) Alexa Fluor® 680 | sc-137093 AF680 | 200 µg/ml | $357.00 | |||
ALAS-H Antibody (F-5) Alexa Fluor® 790 | sc-137093 AF790 | 200 µg/ml | $357.00 |