Date published: 2026-4-24

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AlaRS Antibody (E-9): sc-165991

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Datasheets
  • AlaRS Antibody (E-9) is a mouse monoclonal IgG2a κ, cited in 1 publications, provided at 200 µg/ml
  • raised against amino acids 701-968 mapping at the C-terminus of AlaRS of human origin
  • recommended for detection of AlaRS of mouse, rat and human origin by WB, IP, IF and ELISA
  • m-IgG Fc BP-HRP, m-IgG2a BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for AlaRS Antibody (E-9) for WB applications. These reagents are now offered in bundles with AlaRS Antibody (E-9) (see ordering information below).
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AlaRS Antibody (E-9) is a mouse monoclonal IgG2a antibody that detects alanyl-tRNA synthetase (AlaRS) in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). AlaRS is a crucial enzyme that catalyzes the ATP-dependent attachment of alanine to its corresponding tRNA, forming alanyl-tRNA(Ala), which is essential for protein synthesis. This process ensures accurate translation of genetic information into functional proteins, thereby maintaining cellular integrity and function. AlaRS consists of 968 amino acids and is located in the cytoplasm, where AlaRS operates as a monomer. Proper functioning of AlaRS is critical, as defects in AlaRS gene can lead to misfolded protein accumulation, resulting in cellular stress and potential cell death. Anti-AlaRS antibody (E-9) enables researchers to effectively study AlaRS expression and localization, contributing to a deeper understanding of AlaRS role in cellular processes and implications of AlaRS dysfunction in various diseases.

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

AlaRS Antibody (E-9)

sc-165991
200 µg/ml
$322.00

AlaRS Antibody (E-9): m-IgG Fc BP-HRP Bundle

sc-537427
200 µg Ab; 10 µg BP
$361.00

AlaRS Antibody (E-9): m-IgGκ BP-HRP Bundle

sc-534686
200 µg Ab; 40 µg BP
$361.00

AlaRS Antibody (E-9): m-IgG2a BP-HRP Bundle

sc-548011
200 µg Ab; 10 µg BP
$361.00

What is the recommended fixation method for immunofluorescence staining with AlaRS (E-9): sc-165991 monoclonal antibody?

Asked by: Professor Griffin
Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10°C methanol and allowing to air dry. The full protocol can be viewed here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-03-01
  • y_2026, m_4, d_23, h_7CST
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Rated 5 out of 5 by from Good cytoplasmic staining with HeLaGood cytoplasmic staining with HeLa -SCBT QC
Date published: 2015-02-04
Rated 5 out of 5 by from Strong band at correct mw in MCF7Strong band at correct mw in MCF7, K-562 and Caki-1 whole cell lysates with minimal background bands -SCBT QC
Date published: 2015-01-05
Rated 5 out of 5 by from We wanted to detect alanyl tRNA synthetaseWe wanted to detect alanyl tRNA synthetase in different human cell lines and AlaRS (E9) was our choice of antibody from Santa Cruz. The polyclonal antibody worked really well at 1:2000 dilution (incubated overnight) and it was very specific. Though at higher exposure we saw some lower molecular weight bands appearing but that happens with any antibody. I have also used the antibody for immunofluorescence at 1:200 dilution and it worked well.
Date published: 2014-05-12
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AlaRS Antibody (E-9) is rated 5.0 out of 5 by 3.
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