AlaRS Antibody (A-6): sc-165990

AlaRS (A-6) is a mouse monoclonal IgG2b kappa light chain antibody that detects AlaRS protein of mouse, rat and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP) and enzyme-linked immunosorbent assay (ELISA). Anti-AlaRS antibody (A-6) is available as both non-conjugated form, as well as multiple conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC) and multiple Alexa Fluor® conjugates. Aminoacyl-tRNA synthetases catalyze the aminoacylation of tRNAs by their corresponding amino acids, linking amino acids with tRNA-contained nucleotide triplets. Class II tRNA synthases are a highly conserved subfamily of tRNA synthetases with a catalytic domain for interacting with the amino acid acceptor of the tRNA and a second domain for interacting with the rest of the tRNA molecule. AlaRS (alanyl-tRNA synthetase), also known as AARS, is a 968 amino acid cytoplasmic protein belonging to the class II subfamily of tRNA synthases. AlaRS functions as a monomer, catalyzing the ATP-dependent attachment of alanine to corresponding tRNA(Ala), producing alanyl-tRNA(Ala). Defects in the gene encoding AlaRS may lead to an accumulation of misfolded proteins within the cell, ultimately leading to cell death.

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AlaRS Antibody (A-6) References:

1. Origin of mitochondria in relation to evolutionary history of eukaryotic alanyl-tRNA synthetase.  |  Chihade, JW., et al. 2000. Proc Natl Acad Sci U S A. 97: 12153-7. PMID: 11035802
2. Translocation within the acceptor helix of a major tRNA identity determinant.  |  Lovato, MA., et al. 2001. EMBO J. 20: 4846-53. PMID: 11532948
3. Interaction network of human aminoacyl-tRNA synthetases and subunits of elongation factor 1 complex.  |  Sang Lee, J., et al. 2002. Biochem Biophys Res Commun. 291: 158-64. PMID: 11829477
4. Editing-defective tRNA synthetase causes protein misfolding and neurodegeneration.  |  Lee, JW., et al. 2006. Nature. 443: 50-5. PMID: 16906134
5. Aminoacylation of RNA minihelices with alanine.  |  Francklyn, C. and Schimmel, P. 1989. Nature. 337: 478-81. PMID: 2915692
6. Human alanyl-tRNA synthetase: conservation in evolution of catalytic core and microhelix recognition.  |  Shiba, K., et al. 1995. Biochemistry. 34: 10340-9. PMID: 7654687
7. Wide cross-species aminoacyl-tRNA synthetase replacement in vivo: yeast cytoplasmic alanine enzyme replaced by human polymyositis serum antigen.  |  Ripmaster, TL., et al. 1995. Proc Natl Acad Sci U S A. 92: 4932-6. PMID: 7761427
8. Localization of two human autoantigen genes by PCR screening and in situ hybridization--glycyl-tRNA synthetase locates to 7p15 and alanyl-tRNA synthetase locates to 16q22.  |  Nichols, RC., et al. 1995. Genomics. 30: 131-2. PMID: 8595897

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-AlaRS Antibody (A-6) has 3 citations in a variety of scientific publications.

Primary antibodies like Anti-AlaRS Antibody (A-6) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

AlaRS Antibody (A-6) Specifications

Alternate Product Name: AlaRS

Product Clone Name: A-6

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 701-968

Epitope Target: AlaRS

Epitope Species: human

Product Reactivity/Detection: AlaRS

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 3 citations in various scientific publications

Available Conjugates/Forms for AlaRS Antibody (A-6): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin