AIM2 Antibody (B-8): sc-515514

AIM2 Antibody (B-8) is a mouse monoclonal IgG1 kappa light chain antibody that detects AIM2 of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). AIM2 (B-8) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. AIM2, also known as Interferon-Inducible Protein AIM2 or PYHIN4, is a 343 amino acid protein belonging to the HIN-200 family and plays a crucial role in the immune response by detecting cytosolic DNA, which indicates infection or cellular damage. Upon binding to DNA, AIM2 forms a complex with the adaptor protein ASC, leading to Caspase-1 activation and pro-inflammatory cytokine release, thereby promoting an inflammatory response. This function is particularly important in tumor suppression, as AIM2 represses NFκB transcriptional activity, which is often upregulated in various cancers. Localized primarily in the nucleus, AIM2 contains one DAPIN domain and one HIN-200 domain, with the DAPIN domain facilitating protein-protein interactions and the HIN-200 domain enabling direct DNA binding. AIM2 is expressed at high levels in tissues such as the small intestine, testis, peripheral blood leukocytes, and spleen, and defects in AIM2 have been implicated in microsatellite unstable colon cancers, highlighting its significance in both immune regulation and cancer biology.

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AIM2 Antibody (B-8) References:

1. Biochemical and growth regulatory activities of the HIN-200 family member and putative tumor suppressor protein, AIM2.  |  Cresswell, KS., et al. 2005. Biochem Biophys Res Commun. 326: 417-24. PMID: 15582594
2. AIM2 suppresses human breast cancer cell proliferation in vitro and mammary tumor growth in a mouse model.  |  Chen, IF., et al. 2006. Mol Cancer Ther. 5: 1-7. PMID: 16432157
3. The putative tumor suppressor AIM2 is frequently affected by different genetic alterations in microsatellite unstable colon cancers.  |  Woerner, SM., et al. 2007. Genes Chromosomes Cancer. 46: 1080-9. PMID: 17726700
4. AIM2 recognizes cytosolic dsDNA and forms a caspase-1-activating inflammasome with ASC.  |  Hornung, V., et al. 2009. Nature. 458: 514-8. PMID: 19158675
5. AIM2 activates the inflammasome and cell death in response to cytoplasmic DNA.  |  Fernandes-Alnemri, T., et al. 2009. Nature. 458: 509-13. PMID: 19158676
6. The DNA-sensing AIM2 inflammasome controls radiation-induced cell death and tissue injury.  |  Hu, B., et al. 2016. Science. 354: 765-768. PMID: 27846608
7. AIM2 inflammasome contributes to brain injury and chronic post-stroke cognitive impairment in mice.  |  Kim, H., et al. 2020. Brain Behav Immun. 87: 765-776. PMID: 32201254
8. The AIM2 and NLRP3 inflammasomes trigger IL-1-mediated antitumor effects during radiation.  |  Han, C., et al. 2021. Sci Immunol. 6: PMID: 33963060
9. Perfluoroalkyl substance pollutants activate the innate immune system through the AIM2 inflammasome.  |  Wang, LQ., et al. 2021. Nat Commun. 12: 2915. PMID: 34006824
10. AIM2 promotes renal cell carcinoma progression and sunitinib resistance through FOXO3a-ACSL4 axis-regulated ferroptosis.  |  Wang, Q., et al. 2023. Int J Biol Sci. 19: 1266-1283. PMID: 36923928
11. Pristimerin suppresses AIM2 inflammasome by modulating AIM2-PYCARD/ASC stability via selective autophagy to alleviate tendinopathy.  |  Jiang, H., et al. 2024. Autophagy. 20: 76-93. PMID: 37647255
12. Cloning a novel member of the human interferon-inducible gene family associated with control of tumorigenicity in a model of human melanoma.  |  DeYoung, KL., et al. 1997. Oncogene. 15: 453-7. PMID: 9242382

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-AIM2 Antibody (B-8) has 7 citations in a variety of scientific publications.

Primary antibodies like Anti-AIM2 Antibody (B-8) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

AIM2 Antibody (B-8) Specifications

Alternate Product Name: Absent In Melanoma 2

Product Clone Name: B-8

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 10-179

Epitope Target: AIM2

Epitope Species: human

Product Reactivity/Detection: AIM2

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 7 citations in various scientific publications

Available Conjugates/Forms for AIM2 Antibody (B-8): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin