AF-10 Antibody (HAF10 9A5/2): sc-53156

AF-10 Antibody (HAF10 9A5/2) is a mouse monoclonal IgG2b kappa light chain antibody that detects AF-10 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), and flow cytometry (FCM). AF-10 (HAF10 9A5/2) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. AF-10 protein is a crucial nuclear transcription factor that plays a significant role in gene expression regulation, particularly in acute myeloid leukemia, where AF-10 is implicated in the t(10;11) chromosomal translocation. This translocation leads to fusion proteins that can disrupt normal cellular functions, making AF-10 a key player in oncogenesis. Human AF-10 protein consists of 1,027 amino acids and shares 90% identity with mouse AF-10, which contains 1,061 amino acids. AF-10 is predominantly expressed in testis, highlighting AF-10′s potential role in reproductive biology and development. AF-10′s ability to interact with other transcription factors, such as AF-17, further underscores AF-10′s importance in cellular signaling pathways and gene regulation, making AF-10 a valuable target for research in cancer biology and developmental studies.

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AF-10 Antibody (HAF10 9A5/2) References:

1. Molecular and fluorescence in situ hybridization analysis of a 10;11 rearrangement in a case of infant acute monocytic leukemia.  |  Roll, P., et al. 2002. Cancer Genet Cytogenet. 135: 187-91. PMID: 12127405
2. ALL-1 is a histone methyltransferase that assembles a supercomplex of proteins involved in transcriptional regulation.  |  Nakamura, T., et al. 2002. Mol Cell. 10: 1119-28. PMID: 12453419
3. Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences.  |  Strausberg, RL., et al. 2002. Proc Natl Acad Sci U S A. 99: 16899-903. PMID: 12477932
4. The leucine zipper motif of the Drosophila AF10 homologue can inhibit PRE-mediated repression: implications for leukemogenic activity of human MLL-AF10 fusions.  |  Perrin, L., et al. 2003. Mol Cell Biol. 23: 119-30. PMID: 12482966
5. A novel class of zinc finger/leucine zipper genes identified from the molecular cloning of the t(10;11) translocation in acute leukemia.  |  Chaplin, T., et al. 1995. Blood. 85: 1435-41. PMID: 7888665
6. Alternative splicing in wild-type AF10 and CALM cDNAs and in AF10-CALM and CALM-AF10 fusion cDNAs produced by the t(10;11)(p13-14;q14-q21) suggests a potential role for truncated AF10 polypeptides.  |  Silliman, CC., et al. 1998. Leukemia. 12: 1404-10. PMID: 9737689

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-AF-10 Antibody (HAF10 9A5/2) has 2 citations in a variety of scientific publications.

Primary antibodies like Anti-AF-10 Antibody (HAF10 9A5/2) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

AF-10 Antibody (HAF10 9A5/2) Specifications

Alternate Product Name: AF-10

Product Clone Name: HAF10 9A5/2

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 1055-1069

Epitope Target: AF-10

Epitope Species: human

Product Reactivity/Detection: AF-10

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, FCM

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 2 citations in various scientific publications

Available Conjugates/Forms for AF-10 Antibody (HAF10 9A5/2): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin