ADH5 Antibody (B-1): sc-518202

ADH5 Antibody (B-1) is a mouse monoclonal IgG1 kappa light chain antibody that detects ADH5 of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). ADH5 Antibody (B-1) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. ADH5, also known as alcohol dehydrogenase 5 (class III), formaldehyde dehydrogenase, ADHX, ADH-3, or GSNOR, is a 374 amino acid cytoplasmic protein that plays a crucial role in cellular metabolism by catalyzing the oxidation of various substrates, including long-chain primary alcohols and S-hydroxymethyl-glutathione, which forms from the reaction of formaldehyde with glutathione. This enzymatic activity is vital for detoxifying formaldehyde, a potentially harmful byproduct of various metabolic processes, thereby protecting cells from oxidative stress and maintaining metabolic homeostasis. ADH5 exists as a homodimer and is expressed ubiquitously, utilizing iron as a cofactor in catalytic function. Notably, genetic variations in the ADH5 gene have been linked to differences in drug and alcohol dependence among individuals, highlighting its significance in pharmacogenomics and addiction studies.

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ADH5 Antibody (B-1) References:

1. Human class V alcohol dehydrogenase (ADH5): A complex transcription unit generates C-terminal multiplicity.  |  Strömberg, P. and Höög, JO. 2000. Biochem Biophys Res Commun. 278: 544-9. PMID: 11095947
2. Posttranscriptional regulation of human ADH5/FDH and Myf6 gene expression by upstream AUG codons.  |  Kwon, HS., et al. 2001. Arch Biochem Biophys. 386: 163-71. PMID: 11368338
3. POZ domain transcription factor, FBI-1, represses transcription of ADH5/FDH by interacting with the zinc finger and interfering with DNA binding activity of Sp1.  |  Lee, DK., et al. 2002. J Biol Chem. 277: 26761-8. PMID: 12004059
4. Alcohol dehydrogenase genes: restriction fragment length polymorphisms for ADH4 (pi-ADH) and ADH5 (chi-ADH) and construction of haplotypes among different ADH classes.  |  Edman, K. and Maret, W. 1992. Hum Genet. 90: 395-401. PMID: 1362387
5. Cloning and characterization of the ADH5 gene encoding human alcohol dehydrogenase 5, formaldehyde dehydrogenase.  |  Hur, MW. and Edenberg, HJ. 1992. Gene. 121: 305-11. PMID: 1446828
6. An MspI RFLP in the human ADH5 gene.  |  Edman, K. and Maret, W. 1990. Nucleic Acids Res. 18: 2836. PMID: 1692617
7. The influence of genetic polymorphisms in XRCC3 and ADH5 genes on the frequency of genotoxicity biomarkers in workers exposed to formaldehyde.  |  Ladeira, C., et al. 2013. Environ Mol Mutagen. 54: 213-21. PMID: 23355119
8. Analysis of disease model iPSCs derived from patients with a novel Fanconi anemia-like IBMFS ADH5/ALDH2 deficiency.  |  Mu, A., et al. 2021. Blood. 137: 2021-2032. PMID: 33512438
9. Cord Blood Transplantation Using Myeloablative Conditioning for Pediatric Advanced Myelodysplastic Syndrome in AMeD Syndrome With a Novel ADH5 Variant.  |  Morishita, Y., et al. 2025. Pediatr Blood Cancer. 72: e31465. PMID: 39616414
10. Sp3 and Sp4 can repress transcription by competing with Sp1 for the core cis-elements on the human ADH5/FDH minimal promoter.  |  Kwon, HS., et al. 1999. J Biol Chem. 274: 20-8. PMID: 9867805

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-ADH5 Antibody (B-1) has 0 citations in a variety of scientific publications.

Primary antibodies like Anti-ADH5 Antibody (B-1) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

ADH5 Antibody (B-1) Specifications

Alternate Product Name: ADH5

Product Clone Name: B-1

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 322-347

Epitope Target: ADH5

Epitope Species: human

Product Reactivity/Detection: ADH5

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 0 citations in various scientific publications

Available Conjugates/Forms for ADH5 Antibody (B-1): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin