ADH4 Antibody (F-6): sc-515217

ADH4 Antibody (F-6) is a mouse monoclonal IgG1 kappa light chain antibody that detects ADH4 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). ADH4 Antibody (F-6) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. ADH4, also known as alcohol dehydrogenase 4, is a 380 amino acid enzyme that plays a crucial role in metabolizing diverse substrates, such as retinol, hydroxysteroids, ethanol, aliphatic alcohols, and lipid peroxidation products. ADH4 is primarily localized in the cytoplasm and functions as a homodimer, utilizing zinc as a cofactor to facilitate NAD+-dependent conversion of alcohols into aldehydes or ketones, which is essential for metabolic degradation of alcohols in the body. ADH4 undergoes alternative splicing, resulting in multiple isoforms that may have distinct functional properties. ADH4 gene is located on human chromosome 4, which harbors a cluster of alcohol dehydrogenase genes and represents nearly 6% of the human genome, including some of the largest gene deserts found in human chromosomes. Understanding ADH4 function and regulation is vital, as ADH4 contributes to alcohol metabolism and plays a role in detoxifying various endogenous and exogenous compounds, highlighting ADH4′s importance in both health and disease contexts.

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ADH4 Antibody (F-6) References:

1. Alcohol dehydrogenase genes: restriction fragment length polymorphisms for ADH4 (pi-ADH) and ADH5 (chi-ADH) and construction of haplotypes among different ADH classes.  |  Edman, K. and Maret, W. 1992. Hum Genet. 90: 395-401. PMID: 1362387
2. Association of ADH and ALDH genes with alcohol dependence in the Irish Affected Sib Pair Study of alcohol dependence (IASPSAD) sample.  |  Kuo, PH., et al. 2008. Alcohol Clin Exp Res. 32: 785-95. PMID: 18331377
3. Recessive genetic mode of an ADH4 variant in substance dependence in African-Americans: A model of utility of the HWD test.  |  Luo, X., et al. 2008. Behav Brain Funct. 4: 42. PMID: 18801187
4. Cloning and characterization of the human ADH4 gene.  |  von Bahr-Lindström, H., et al. 1991. Gene. 103: 269-74. PMID: 1889753
5. Associations of ADH and ALDH2 gene variation with self report alcohol reactions, consumption and dependence: an integrated analysis.  |  Macgregor, S., et al. 2009. Hum Mol Genet. 18: 580-93. PMID: 18996923
6. Polymorphisms in the promoter region of the human class II alcohol dehydrogenase (ADH4) gene affect both transcriptional activity and ethanol metabolism in Japanese subjects.  |  Kimura, Y., et al. 2009. J Toxicol Sci. 34: 89-97. PMID: 19182438
7. ADH single nucleotide polymorphism associations with alcohol metabolism in vivo.  |  Birley, AJ., et al. 2009. Hum Mol Genet. 18: 1533-42. PMID: 19193628
8. Isolation of pi-alcohol dehydrogenase of human liver: is it a determinant of alcoholism?  |  Li, TK., et al. 1977. Proc Natl Acad Sci U S A. 74: 4378-81. PMID: 270680
9. Human class II (pi) alcohol dehydrogenase has a redox-specific function in norepinephrine metabolism.  |  Mårdh, G., et al. 1986. Proc Natl Acad Sci U S A. 83: 8908-12. PMID: 3466164

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-ADH4 Antibody (F-6) has 1 citations in a variety of scientific publications.

Primary antibodies like Anti-ADH4 Antibody (F-6) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

ADH4 Antibody (F-6) Specifications

Alternate Product Name: ADH4

Product Clone Name: F-6

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 36-75

Epitope Target: ADH4

Epitope Species: human

Product Reactivity/Detection: ADH4

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 1 citations in various scientific publications

Available Conjugates/Forms for ADH4 Antibody (F-6): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin