Adenovirus-2/5 E1A Antibody (A-3): sc-25292

Adenovirus-2/5 E1A Antibody (A-3) is a mouse monoclonal IgG1 antibody that detects E1A protein of Adenovirus-2 and Adenovirus-5 by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-E1A antibody (A-3) targets the early region (E1) of the adenovirus genome, which is crucial for the virus′s ability to transform host cells. E1A protein plays a significant role in regulating the cell cycle by interacting with key cellular proteins, including retinoblastoma (Rb) protein and cyclin A, which are vital for controlling cell proliferation. By binding to Rb, E1A disrupts its function, leading to the release of E2F transcription factors that promote the expression of genes necessary for DNA synthesis and cell division. This mechanism is essential for the adenovirus to hijack the host′s cellular machinery, allowing for viral replication and the potential for oncogenic transformation. E1A′s ability to modulate cellular gene expression not only facilitates viral infection but also provides insights into the molecular pathways involved in cancer development, making the study of E1A and its interactions with cellular proteins a significant area of research in virology and oncology.

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Adenovirus-2/5 E1A Antibody (A-3) References:

1. Independent transformation activity by adenovirus-5 E1A-conserved regions 1 or 2 mutants.  |  Svensson, C., et al. 1991. Virology. 182: 553-61. PMID: 1827227
2. Comparative analysis of ovine adenovirus 287 and human adenovirus 2 and 5 based on their codon usage.  |  Gu, YX., et al. 2012. DNA Cell Biol. 31: 360-6. PMID: 21810025
3. Hepatocyte Heparan Sulfate Is Required for Adeno-Associated Virus 2 but Dispensable for Adenovirus 5 Liver Transduction In Vivo.  |  Zaiss, AK., et al. 2016. J Virol. 90: 412-20. PMID: 26491162
4. Adenovirus 5 E1A-Mediated Suppression of p53 via FUBP1.  |  Frost, JR., et al. 2018. J Virol. 92: PMID: 29743362
5. Incorporation of 5-bromodeoxycytidine in the adenovirus 2 replication origin interferes with nuclear factor 1 binding.  |  de Vries, E., et al. 1987. Nucleic Acids Res. 15: 7223-34. PMID: 3658692
6. Review of Safety Data for Adenovirus 5 as a Delivery Vector for Intratumoral Cancer Gene Therapy.  |  Sheldon, Y., et al. 2023. Hum Gene Ther. 34: 314-324. PMID: 36851898
7. Enhanced deoxyribonucleic acid polymerase activity in human embryonic kidney cultures infected with adenovirus 2 or 12.  |  Ledinko, N. 1968. J Virol. 2: 89-98. PMID: 5742041
8. Nucleotide sequences and mapping of novel heterogenous 5'-termini of adenovirus 2 early region 4 mRNA.  |  Hashimoto, S., et al. 1981. Nucleic Acids Res. 9: 1675-89. PMID: 6164992
9. An amazing sequence arrangement at the 5' ends of adenovirus 2 messenger RNA.  |  Chow, LT., et al. 1977. Cell. 12: 1-8. PMID: 902310
10. Studies of low molecular weight RNA from cells infected with adenovirus 2. II. Heterogeneity at the 5' end of VA-RNA I.  |  Celma, ML., et al. 1977. J Biol Chem. 252: 9043-6. PMID: 925035

The preferred Adenovirus-5 E1A antibody is Adenovirus-5 E1A Antibody (M58): sc-58658.