Adenovirus-5 E1A Antibody (M58): sc-58658

Adenovirus-5 E1A Antibody (M58) is a mouse monoclonal IgG2a kappa light chain antibody that detects Adenovirus-5 E1A, with species reactivity in non-species reactive (NSR) samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry. Anti-Adenovirus-5 E1A antibody (M58) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. E1A plays a crucial role in early stages of adenoviral infection, as E1A is essential for transformation and immortalization of primary cells. Specifically, E1A interacts with retinoblastoma protein (pRb) and cyclin A, forming complexes that regulate cell cycle and transcription of other viral genes. This interaction allows viral hijacking of host cell machinery, promoting viral replication and pathogenesis. E1A′s ability to modulate transcription of specific cellular genes underscores E1A′s importance in both viral biology and potential therapeutic applications, making Adenovirus-5 E1A (M58) monoclonal antibody an invaluable tool for researchers studying adenoviral infections and host cellular processes.

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Adenovirus-5 E1A Antibody (M58) References:

1. Relaxed template specificity in fowl adenovirus 1 DNA replication initiation.  |  Rademaker, HJ., et al. 2006. J Gen Virol. 87: 553-562. PMID: 16476976
2. Isolation of the human cdk2 gene that encodes the cyclin A- and adenovirus E1A-associated p33 kinase.  |  Tsai, LH., et al. 1991. Nature. 353: 174-7. PMID: 1653904
3. Cell cycle regulation of histone H1 kinase activity associated with the adenoviral protein E1A.  |  Giordano, A., et al. 1991. Science. 253: 1271-5. PMID: 1653969
4. Equine adenovirus 1 infection of hospitalised and healthy foals and horses.  |  Bell, SA., et al. 2006. Equine Vet J. 38: 379-81. PMID: 16866210
5. An adenovirus type 5 early gene function regulates expression of other early viral genes.  |  Jones, N. and Shenk, T. 1979. Proc Natl Acad Sci U S A. 76: 3665-9. PMID: 291030
6. Association between an oncogene and an anti-oncogene: the adenovirus E1A proteins bind to the retinoblastoma gene product.  |  Whyte, P., et al. 1988. Nature. 334: 124-9. PMID: 2968522
7. Monoclonal antibodies specific for adenovirus early region 1A proteins: extensive heterogeneity in early region 1A products.  |  Harlow, E., et al. 1985. J Virol. 55: 533-46. PMID: 3894685
8. Viral DNA in transformed cells. II. A study of the sequences of adenovirus 2 DNA IN NINE LINES OF TRANSFORMED RAT CELLS USING SPECIFIC FRAGMENTS OF THE VIRAL GENOME;  |  Gallimore, PH. 1974. J Mol Biol. 89: 49-72. PMID: 4613857
9. Pre-early adenovirus 5 gene product regulates synthesis of early viral messenger RNAs.  |  Berk, AJ., et al. 1979. Cell. 17: 935-44. PMID: 487437

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Adenovirus-5 E1A Antibody (M58) has 17 citations in a variety of scientific publications.

Primary antibodies for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Adenovirus-5 E1A Antibody (M58) Specifications

Alternate Product Name: Adenovirus-5 E1A

Product Clone Name: M58

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids:

Epitope Target: Adenovirus-5 E1A

Epitope Species: Adenovirus-5 E1A

Product Reactivity/Detection: Adenovirus-5 E1A

Species Reactivity/Detection: NSR

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P)

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 17 citations in various scientific publications

Available Conjugates/Forms for Adenovirus-5 E1A Antibody (M58): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin