Adducin α Antibody (4D1): sc-33633

Adducin α Antibody (4D1) is a mouse monoclonal IgG1 kappa light chain antibody that detects Adducin alpha protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry. Anti-Adducin α antibody (4D1) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Adducins are a family of cytoskeletal proteins encoded by three genes (α, β, γ), and Adducin α plays a crucial role in organizing the cortical actin cytoskeletal network, which maintains cell shape and integrity. Adducin α is primarily localized at the spectrin-actin junction of the plasma membrane, where Adducin α contributes to structural stability and participates in cell signaling and motility. Adducin α′s importance extends to its association with sodium-pump activity in the renal tubule, linking Adducin α to hypertension, a significant health concern. While Adducin α and Adducin γ exhibit ubiquitous expression, Adducin β is predominantly found in the brain and hematopoietic tissues, highlighting the diverse roles of these proteins in different cellular contexts.

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Adducin α Antibody (4D1) References:

1. Targeted disruption of the beta adducin gene (Add2) causes red blood cell spherocytosis in mice.  |  Gilligan, DM., et al. 1999. Proc Natl Acad Sci U S A. 96: 10717-22. PMID: 10485892
2. Linkage but lack of association for blood pressure and the alpha-adducin locus in normotensive twins.  |  Busjahn, A., et al. 1999. J Hypertens. 17: 1437-41. PMID: 10526904
3. Association of the alpha-adducin polymorphism with blood pressure and risk of myocardial infarction.  |  Psaty, BM., et al. 2000. J Hum Hypertens. 14: 95-7. PMID: 10723114
4. Cleavage of the actin-capping protein alpha -adducin at Asp-Asp-Ser-Asp633-Ala by caspase-3 is preceded by its phosphorylation on serine 726 in cisplatin-induced apoptosis of renal epithelial cells.  |  van de Water, B., et al. 2000. J Biol Chem. 275: 25805-13. PMID: 10823823
5. Mild spherocytic hereditary elliptocytosis and altered levels of alpha- and gamma-adducins in beta-adducin-deficient mice.  |  Muro, AF., et al. 2000. Blood. 95: 3978-85. PMID: 10845937
6. Hypertension-linked mutation in the adducin alpha-subunit leads to higher AP2-mu2 phosphorylation and impaired Na+,K+-ATPase trafficking in response to GPCR signals and intracellular sodium.  |  Efendiev, R., et al. 2004. Circ Res. 95: 1100-8. PMID: 15528469
7. Hypertension: molecular approach.  |  Lele, RD. 2004. J Assoc Physicians India. 52: 53-62. PMID: 15633721
8. Profiling estrogen-regulated gene expression changes in normal and malignant human ovarian surface epithelial cells.  |  Syed, V., et al. 2005. Oncogene. 24: 8128-43. PMID: 16116479
9. Transcriptional profiling of C2C12 myotubes in response to SHIP2 depletion and insulin stimulation.  |  Huard, C., et al. 2007. Genomics. 89: 270-9. PMID: 17123777
10. Primary structure and domain organization of human alpha and beta adducin.  |  Joshi, R., et al. 1991. J Cell Biol. 115: 665-75. PMID: 1840603
11. Hypertension-associated point mutations in the adducin alpha and beta subunits affect actin cytoskeleton and ion transport.  |  Tripodi, G., et al. 1996. J Clin Invest. 97: 2815-22. PMID: 8675693
12. Rabphilin-3A: a multifunctional regulator of synaptic vesicle traffic.  |  Burns, ME., et al. 1998. J Gen Physiol. 111: 243-55. PMID: 9450942

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Adducin α Antibody (4D1) has 1 citations in a variety of scientific publications.

Primary antibodies like Anti-Adducin α Antibody (4D1) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Adducin α Antibody (4D1) Specifications

Alternate Product Name: ADD1

Product Clone Name: 4D1

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 181-245

Epitope Target: Adducin α

Epitope Species: rat

Product Reactivity/Detection: Adducin α

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P)

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 1 citations in various scientific publications

Available Conjugates/Forms for Adducin α Antibody (4D1): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin