ADAR1 Antibody (D-8) recommended for detection of all ADAR1 isoforms of human origin by WB, IP, IF, IHC(P) and ELISA

ADAR1 Antibody (D-8): sc-271854

ADAR1 Antibody (D-8) is rated 4.5 out of 5 by 11.
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Datasheets

    • Anti-ADAR1 Antibody (D-8) is a mouse monoclonal IgG1 (lambda light chain) ADAR1 antibody provided at 200 µg/ml
    • raised against amino acids 1051-1226 mapping at the C-terminus of ADAR1 of human origin
    • ADAR1 Antibody (D-8) is recommended for detection of all ADAR1 isoforms of human origin by WB, IP, IF, IHC(P) and ELISA
    • Anti-ADAR1 Antibody (D-8) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGλ BP-HRP (mouse IgGλ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of ADAR1 (D-8): sc-271854.

Can ADAR1 (D-8): sc-271854 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: Trav11
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, ADAR1 (D-8): sc-271854 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from ADAR1 Western blot(HEK293细胞) 我是用来跑HEK293细胞的蛋白样品,Western blot的结果,特异性条带很好,没什么非特异性的条带的产生。
Date published: 2018-04-19
Rated 5 out of 5 by from 抗体不错 抗体很好用,申请后,邮寄很及时,并且该抗体在1:1000的稀释比例下,进行western blot实验,条带清晰可见!
Date published: 2017-12-08
Rated 5 out of 5 by from Nice antibody for WB I diluted this antibody at 1:200 for western blot. A strong band at the predicted size of ADAR1 was detected.
Date published: 2017-11-21
Rated 4 out of 5 by from works well works well for western (1:1000) in bone marrow derived macrophages.
Date published: 2017-10-19
Rated 1 out of 5 by from Did not work well for me A small sample I asked for free earlier seemed to work well. However, with the D-8 I ordered, I could not detect adar1. It also have a strong off target.
Date published: 2017-08-06
Rated 5 out of 5 by from Great Antibody for IHC This antibody is well-cited for a variety of applications including WB, IP, IF, and even IHC and ELISA. Strong, clear signal with little to no background. Beautiful tissue staining.
Date published: 2017-01-23
Rated 4 out of 5 by from good antibody for IF works well for IF (1:100) in U2OS cells. not tested in other applications yet.
Date published: 2016-12-14
Rated 5 out of 5 by from Positive results in western blot analysis Positive results in western blot analysis of ADAR1 expression in NTERA-cl.D1, U-2 OS and Ramos whole cell lysates and HeLa and K-562 nuclear extracts. -SCBT QC
Date published: 2015-05-10
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