ADAM10 Antibody (A-3) recommended for detection of ADAM10 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

ADAM10 Antibody (A-3): sc-48400

ADAM10 Antibody (A-3) is rated 4.7 out of 5 by 6.
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Datasheets

    • ADAM10 Antibody (A-3) is a mouse monoclonal IgG2a (kappa light chain) provided at 200 µg/ml
    • raised against amino acids 1-300 of ADAM10 of human origin
    • Anti-ADAM10 Antibody (A-3) is recommended for detection of ADAM10 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
    • Anti-ADAM10 Antibody (A-3) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of ADAM10 (A-3): sc-48400.
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What is the recommended secondary reagent to be used with this mouse monoclonal primary?

Asked by: jerojero
We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 15
Date published: 2017-02-13
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Rated 5 out of 5 by from Great antibody, clear bands in western blot. I received a sample of this antibody (10ug/50ul). I tested it on protein extracts (30ug) of a cell line of trophoblasts induced to differentiate to an endothelial-like phenotype. First antibody dilution: 1: 1000 (0.2 ug/ml) Dilution second antibody (anti mouse - HRP): 1: 5000 Detection: ECL Exposure time: 45 sec. Approx.
Date published: 2018-05-14
Rated 3 out of 5 by from Casi no tiene sensibilidad Probamos el anticuerpo para Citometría de flujo y casi no da señal en células que sabemos que tienen ADAM10
Date published: 2018-02-09
Rated 5 out of 5 by from Good for your WB Best used for WB as it will provide clear bands with a strong signal at the appropriate weight. This antibody has been used to publish multiple times.
Date published: 2017-02-02
Rated 5 out of 5 by from This antibody was used in a publication for This antibody was used in a publication for WB on human SK-BR-3 - SCBT Publication Review
Date published: 2015-04-19
Rated 5 out of 5 by from Successful application of this antibody in Successful application of this antibody in WB using mouse brain -SCBT Publication Review
Date published: 2015-03-12
Rated 5 out of 5 by from Publishable IF data with human MDA Publishable IF data with human MDA-MB-231 cells - SCBT Publication Review
Date published: 2014-10-30
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