ACTH/CLIP Antibody (F-3): sc-373878

ACTH/CLIP Antibody (F-3) is a mouse monoclonal IgG2b kappa light chain antibody that specifically binds to an epitope within an internal region of Pro-opiomelanocortin (POMC), also known as corticotropin-lipotropin. POMC is a 267 amino acid polypeptide precursor synthesized in the anterior pituitary gland and other tissues, featuring a multi-domain structure that contains sequences for several peptide hormones within a single polypeptide chain. This structural organization is crucial because it allows POMC to be enzymatically cleaved by prohormone convertases into multiple active peptides, including Adrenocorticotropic Hormone (ACTH, also known as corticotropin) and Corticotropin-like Intermediate Peptide (CLIP). The precise cleavage of POMC is essential for the proper regulation of stress response, metabolism, and pigmentation. ACTH is a 39 amino acid peptide that stimulates cortisol secretion from the adrenal cortex, playing a vital role in stress response and metabolic processes. CLIP is a 21 amino acid neuropeptide secreted by corticotrope cells of the adenohypophysis, involved in various neurological functions. Mutations in the POMC gene can lead to POMC deficiency, characterized by red hair, adrenal insufficiency, and increased susceptibility to obesity. POMC (F-3) monoclonal antibody reacts with POMC and its processed peptides ACTH and CLIP from mouse, rat, and human origins, and is validated for use in western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA) applications.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

ACTH/CLIP Antibody (F-3) References:

1. Differential effects of alpha-, beta- and gamma(2)-melanocyte-stimulating hormones on hypothalamic neuronal activation and feeding in the fasted rat.  |  Millington, GW., et al. 2001. Neuroscience. 108: 437-45. PMID: 11738258
2. Hormone and glucose signalling in POMC and AgRP neurons.  |  Belgardt, BF., et al. 2009. J Physiol. 587: 5305-14. PMID: 19770186
3. The melanocortin system in articular chondrocytes: melanocortin receptors, pro-opiomelanocortin, precursor proteases, and a regulatory effect of alpha-melanocyte-stimulating hormone on proinflammatory cytokines and extracellular matrix components.  |  Grässel, S., et al. 2009. Arthritis Rheum. 60: 3017-27. PMID: 19790046
4. Growth inhibition of thyroid follicular cell-derived cancers by the opioid growth factor (OGF) - opioid growth factor receptor (OGFr) axis.  |  McLaughlin, PJ., et al. 2009. BMC Cancer. 9: 369. PMID: 19835629
5. NF-kappaB activation in hypothalamic pro-opiomelanocortin neurons is essential in illness- and leptin-induced anorexia.  |  Jang, PG., et al. 2010. J Biol Chem. 285: 9706-9715. PMID: 20097762
6. Dephosphorylation/inactivation of tyrosine hydroxylase at the median eminence of the hypothalamus is required for suckling-induced prolactin and adrenocorticotrop hormone responses.  |  Fehér, P., et al. 2010. Brain Res Bull. 82: 141-5. PMID: 20170714
7. Comprehensive analysis of common genetic variation in 61 genes related to steroid hormone and insulin-like growth factor-I metabolism and breast cancer risk in the NCI breast and prostate cancer cohort consortium.  |  Canzian, F., et al. 2010. Hum Mol Genet. 19: 3873-84. PMID: 20634197
8. Peroxisomal localization of the proopiomelanocortin-derived peptides beta-lipotropin and beta-endorphin.  |  Höftberger, R., et al. 2010. Endocrinology. 151: 4801-10. PMID: 20810565

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-ACTH/CLIP Antibody (F-3) has 2 citations in a variety of scientific publications.

Primary antibodies like Anti-ACTH/CLIP Antibody (F-3) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

ACTH/CLIP Antibody (F-3) Specifications

Alternate Product Name: ACTH/CLIP

Product Clone Name: F-3

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids:

Epitope Target: POMC

Epitope Species: mouse

Product Reactivity/Detection: POMC and the processed active peptides ACTH and CLIP

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 2 citations in various scientific publications

Available Conjugates/Forms for ACTH/CLIP Antibody (F-3): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin

The preferred ACTH antibody is ACTH Antibody (2F6): sc-69648.