ACTH Antibody (2F6): sc-69648

ACTH Antibody (2F6) is a mouse monoclonal IgG1 kappa light chain antibody specifically designed to detect adrenocorticotropic hormone (ACTH), a crucial peptide hormone derived from the precursor protein pro-opiomelanocortin (POMC). Targeting amino acids 1-24 at the N-terminus of human ACTH, anti-ACTH antibody (2F6) effectively detects both POMC and the processed active peptide ACTH in mouse, rat, and human samples using techniques such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry with paraffin-embedded sections (IHCP). ACTH, also known as corticotropin, is a 39-amino-acid peptide produced by the anterior pituitary gland in response to stress, stimulating the secretion of cortisol from the adrenal cortex. Post-translational modifications of POMC, particularly cleavage by prohormone convertases, are essential for generating ACTH and other active peptides like α-MSH and β-endorphin, which play significant roles in stress response, metabolism, and immune function. These modifications are critical because any abnormalities can lead to endocrine disorders such as Cushing′s disease or Addison′s disease. Mutations in the POMC gene can result in POMC deficiency, characterized by adrenal insufficiency, obesity, and altered pigmentation like red hair. Understanding the processing and function of ACTH is vital for researching various metabolic and stress-related disorders. ACTH (2F6) antibody provides researchers with a reliable tool to investigate the expression, processing, and function of ACTH and POMC, facilitating advancements in endocrinology and neurobiology.

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ACTH Antibody (2F6) References:

1. Differential effects of alpha-, beta- and gamma(2)-melanocyte-stimulating hormones on hypothalamic neuronal activation and feeding in the fasted rat.  |  Millington, GW., et al. 2001. Neuroscience. 108: 437-45. PMID: 11738258
2. Hormone and glucose signalling in POMC and AgRP neurons.  |  Belgardt, BF., et al. 2009. J Physiol. 587: 5305-14. PMID: 19770186
3. The melanocortin system in articular chondrocytes: melanocortin receptors, pro-opiomelanocortin, precursor proteases, and a regulatory effect of alpha-melanocyte-stimulating hormone on proinflammatory cytokines and extracellular matrix components.  |  Grässel, S., et al. 2009. Arthritis Rheum. 60: 3017-27. PMID: 19790046
4. Growth inhibition of thyroid follicular cell-derived cancers by the opioid growth factor (OGF) - opioid growth factor receptor (OGFr) axis.  |  McLaughlin, PJ., et al. 2009. BMC Cancer. 9: 369. PMID: 19835629
5. NF-kappaB activation in hypothalamic pro-opiomelanocortin neurons is essential in illness- and leptin-induced anorexia.  |  Jang, PG., et al. 2010. J Biol Chem. 285: 9706-9715. PMID: 20097762
6. Dephosphorylation/inactivation of tyrosine hydroxylase at the median eminence of the hypothalamus is required for suckling-induced prolactin and adrenocorticotrop hormone responses.  |  Fehér, P., et al. 2010. Brain Res Bull. 82: 141-5. PMID: 20170714
7. Comprehensive analysis of common genetic variation in 61 genes related to steroid hormone and insulin-like growth factor-I metabolism and breast cancer risk in the NCI breast and prostate cancer cohort consortium.  |  Canzian, F., et al. 2010. Hum Mol Genet. 19: 3873-84. PMID: 20634197
8. Peroxisomal localization of the proopiomelanocortin-derived peptides beta-lipotropin and beta-endorphin.  |  Höftberger, R., et al. 2010. Endocrinology. 151: 4801-10. PMID: 20810565

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-ACTH Antibody (2F6) has 2 citations in a variety of scientific publications.

Primary antibodies like Anti-ACTH Antibody (2F6) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

ACTH Antibody (2F6) Specifications

Alternate Product Name: ACTH

Product Clone Name: 2F6

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 1-24

Epitope Target: ACTH

Epitope Species: human

Product Reactivity/Detection: POMC and the processed active peptide ACTH

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P)

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 2 citations in various scientific publications

Available Conjugates/Forms for ACTH Antibody (2F6): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin