ACOT1/2 Antibody (F-6): sc-398505

ACOT1/2 Antibody (F-6) is a mouse monoclonal IgG2a antibody that detects acyl-CoA thioesterase 1/2 (ACOT1/2) in human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). ACOT1/2 plays a crucial role in lipid metabolism by catalyzing the hydrolysis of acyl-CoA to produce coenzyme A and free fatty acids, thereby regulating intracellular levels of fatty acids and acyl-CoAs, which is vital for maintaining cellular energy homeostasis and signaling pathways. Notably, ACOT1 is predominantly found in the cytoplasm as a monomer, while ACOT2 is primarily localized in the mitochondria, highlighting their distinct cellular functions and regulatory mechanisms. The ability of ACOT2 to exist in multiple alternatively spliced isoforms adds another layer of complexity to its function, allowing for diverse regulatory roles in fatty acid metabolism. Both ACOT1 and ACOT2 are encoded by genes located on human chromosome 14, underscoring their evolutionary significance and potential implications in metabolic disorders. ACOT1/2 (F-6) antibody is an essential tool for researchers investigating the intricate roles of these enzymes in cellular metabolism and disease.

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ACOT1/2 Antibody (F-6) References:

1. Analysis of the mouse and human acyl-CoA thioesterase (ACOT) gene clusters shows that convergent, functional evolution results in a reduced number of human peroxisomal ACOTs.  |  Hunt, MC., et al. 2006. FASEB J. 20: 1855-64. PMID: 16940157
2. Crystal structure of human mitochondrial acyl-CoA thioesterase (ACOT2).  |  Mandel, CR., et al. 2009. Biochem Biophys Res Commun. 385: 630-3. PMID: 19497300
3. Acyl-CoA thioesterase-2 facilitates mitochondrial fatty acid oxidation in the liver.  |  Moffat, C., et al. 2014. J Lipid Res. 55: 2458-70. PMID: 25114170
4. Multiple mitochondrial thioesterases have distinct tissue and substrate specificity and CoA regulation, suggesting unique functional roles.  |  Bekeova, C., et al. 2019. J Biol Chem. 294: 19034-19047. PMID: 31676684
5. High Expression of ACOT2 Predicts Worse Overall Survival and Abnormal Lipid Metabolism: A Potential Target for Acute Myeloid Leukemia.  |  Yin, X., et al. 2022. J Healthc Eng. 2022: 2669114. PMID: 36193167
6. Acyl-CoA thioesterase-2 facilitates β-oxidation in glycolytic skeletal muscle in a lipid supply dependent manner.  |  Bekeova, C., et al. 2023. bioRxiv.. PMID: 37425757
7. Systematic transcriptome-wide meta-analysis across endocrine disrupting chemicals reveals shared and unique liver pathways, gene networks, and disease associations.  |  Zamora, Z., et al. 2024. Environ Int. 183: 108339. PMID: 38043319
8. Host serine protease ACOT2 assists DENV proliferation by hydrolyzing viral polyproteins.  |  Ma, S., et al. 2024. mSystems. 9: e0097323. PMID: 38112462
9. Transcriptomic evaluation of skin tape-strips in children with allergic asthma uncovers epidermal barrier dysfunction and asthma-associated biomarkers abnormalities.  |  Del Duca, E., et al. 2024. Allergy. 79: 1516-1530. PMID: 38375886
10. Transcriptome-wide association study and Mendelian randomization in pancreatic cancer identifies susceptibility genes and causal relationships with type 2 diabetes and venous thromboembolism.  |  Tan, MCB., et al. 2024. EBioMedicine. 106: 105233. PMID: 39002386

The preferred ACOT1/2 antibody is ACOT1/2 Antibody (F-2): sc-373917.