ACO2 Antibody (775F2I): sc-517651

ACO2 Antibody (775F2I) is a mouse monoclonal IgG1 antibody that detects ACO2 protein of human origin by western blotting (WB). Anti-ACO2 antibody (775F2I) is available as a non-conjugated monoclonal isotype antibody. ACO2, also known as aconitate hydratase, citrate hydrolyase, or aconitase, plays a crucial role in cellular metabolism by catalyzing the reversible conversion of citrate to isocitrate within the tricarboxylic acid cycle, a key pathway for energy production. This enzyme is predominantly located in the mitochondria, where ACO2 helps maintain a critical citrate:isocitrate ratio, which is essential for various metabolic processes. Proper functioning of ACO2 is vital, as ACO2 not only influences energy production but also impacts the regulation of metabolic intermediates that can affect cell signaling and growth. Notably, ACO2 contains a redox-sensitive iron-sulfur cluster that exists in two states—active (Fe4S4) and inactive (Fe3S4)—and ACO2 activity is modulated by the presence of conserved cysteine residues. In normal prostate epithelial cells, high zinc levels inhibit ACO2 activity, leading to an accumulation of citrate and a significantly altered citrate:isocitrate ratio. Conversely, in malignant prostate cells, the inability to accumulate zinc allows ACO2 to regain activity, facilitating citrate oxidation. This dynamic regulation of ACO2 is critical for understanding ACO2′s role in cancer metabolism and potential therapeutic targets.

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ACO2 Antibody (775F2I) References:

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9. Dominant ACO2 mutations are a frequent cause of isolated optic atrophy.  |  Charif, M., et al. 2021. Brain Commun. 3: fcab063. PMID: 34056600
10. Mitochondrial Aconitase ACO2 Links Iron Homeostasis with Tumorigenicity in Non-Small Cell Lung Cancer.  |  Mirhadi, S., et al. 2023. Mol Cancer Res. 21: 36-50. PMID: 36214668
11. ACO2 deficiency increases vulnerability to Parkinson's disease via dysregulating mitochondrial function and histone acetylation-mediated transcription of autophagy genes.  |  Zhu, J., et al. 2023. Commun Biol. 6: 1201. PMID: 38007539
12. Inhibition of hemoglobin expression by heterologous production of nitric oxide synthase in the K562 erythroleukemic cell line.  |  Rafferty, SP., et al. 1996. Blood. 88: 1070-8. PMID: 8704216