ACAP2 Antibody (F-8): sc-376150
- ACAP2 Antibody (F-8) is a mouse monoclonal IgG1 κ ACAP2 antibody, cited in 1 publications, provided at 200 µg/ml
- raised against amino acids 499-619 mapping near the C-terminal of ACAP2 of mouse origin
- ACAP2 Antibody (F-8) is recommended for detection of ACAP2 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
- Anti-ACAP2 Antibody (F-8) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for ACAP2 Antibody (F-8) for WB and IHC(P) applications. These reagents are now offered in bundles with ACAP2 Antibody (F-8) (see ordering information below).
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ACAP2 Antibody (F-8) is a mouse monoclonal IgG1 kappa light chain antibody that detects ACAP2 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). ACAP2 (F-8) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. ACAP2 plays a crucial role in regulating vesicular transport and cytoskeletal organization, which are essential for maintaining cellular structure and function. As a member of the centaurin GTPase-activating protein family, ACAP2 helps transduce phosphoinositide 3-kinase activation into the control of ADP-ribosylation factor-dependent pathways, influencing membrane trafficking processes. ACAP2 interacts with ARF1 and ARF6, while phosphatidylinositol 4,5-bisphosphate modulates ACAP2′s GAP activity, highlighting ACAP2′s importance in cellular signaling. When overexpressed, ACAP2 blocks the formation of ARF6-dependent cellular protrusions, indicating a regulatory role in cell morphology. ACAP2 also interacts with K1L, a protein necessary for Vaccinia Virus growth, through ankyrin repeats, suggesting potential involvement in viral pathogenesis.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
ACAP2 Antibody (F-8) References:
- ACAPs are arf6 GTPase-activating proteins that function in the cell periphery. | Jackson, TR., et al. 2000. J Cell Biol. 151: 627-38. PMID: 11062263
- DEF-1/ASAP1 is a GTPase-activating protein (GAP) for ARF1 that enhances cell motility through a GAP-dependent mechanism. | Furman, C., et al. 2002. J Biol Chem. 277: 7962-9. PMID: 11773070
- Vaccinia virus K1L protein mediates host-range function in RK-13 cells via ankyrin repeat and may interact with a cellular GTPase-activating protein. | Bradley, RR. and Terajima, M. 2005. Virus Res. 114: 104-12. PMID: 16039000
- Vaccinia virus K1L protein supports viral replication in human and rabbit cells through a cell-type-specific set of its ankyrin repeat residues that are distinct from its binding site for ACAP2. | Meng, X. and Xiang, Y. 2006. Virology. 353: 220-33. PMID: 16806385
- GEP100/BRAG2: activator of ADP-ribosylation factor 6 for regulation of cell adhesion and actin cytoskeleton via E-cadherin and alpha-catenin. | Hiroi, T., et al. 2006. Proc Natl Acad Sci U S A. 103: 10672-7. PMID: 16807291
- NMR structural studies of the myristoylated N-terminus of ADP ribosylation factor 6 (Arf6). | Gizachew, D. and Oswald, R. 2006. FEBS Lett. 580: 4296-301. PMID: 16839550
- The arf6 GAP centaurin alpha-1 is a neuronal actin-binding protein which also functions via GAP-independent activity to regulate the actin cytoskeleton. | Thacker, E., et al. 2004. Eur J Cell Biol. 83: 541-54. PMID: 15679100
- An effector domain mutant of Arf6 implicates phospholipase D in endosomal membrane recycling. | Jovanovic, OA., et al. 2006. Mol Biol Cell. 17: 327-35. PMID: 16280360
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ACAP2 Antibody (F-8) | sc-376150 | 200 µg/ml | $316.00 | |||
ACAP2 Antibody (F-8): m-IgG Fc BP-HRP Bundle | sc-527514 | 200 µg Ab; 10 µg BP | $354.00 | |||
ACAP2 Antibody (F-8): m-IgG1 BP-HRP Bundle | sc-532887 | 200 µg Ab; 20 µg BP | $354.00 | |||
ACAP2 Antibody (F-8) AC | sc-376150 AC | 500 µg/ml, 25% agarose | $416.00 | |||
ACAP2 Antibody (F-8) HRP | sc-376150 HRP | 200 µg/ml | $316.00 | |||
ACAP2 Antibody (F-8) FITC | sc-376150 FITC | 200 µg/ml | $330.00 | |||
ACAP2 Antibody (F-8) PE | sc-376150 PE | 200 µg/ml | $343.00 | |||
ACAP2 Antibody (F-8) Alexa Fluor® 488 | sc-376150 AF488 | 200 µg/ml | $357.00 | |||
ACAP2 Antibody (F-8) Alexa Fluor® 546 | sc-376150 AF546 | 200 µg/ml | $357.00 | |||
ACAP2 Antibody (F-8) Alexa Fluor® 594 | sc-376150 AF594 | 200 µg/ml | $357.00 | |||
ACAP2 Antibody (F-8) Alexa Fluor® 647 | sc-376150 AF647 | 200 µg/ml | $357.00 | |||
ACAP2 Antibody (F-8) Alexa Fluor® 680 | sc-376150 AF680 | 200 µg/ml | $357.00 | |||
ACAP2 Antibody (F-8) Alexa Fluor® 790 | sc-376150 AF790 | 200 µg/ml | $357.00 |