ACAD-8 Antibody (D-5): sc-390038
- ACAD-8 Antibody (D-5) is a mouse monoclonal IgG1 κ ACAD-8 antibody provided at 200 µg/ml
- raised against amino acids 251-415 mapping at the C-terminus of ACAD-8 of human origin
- ACAD-8 Antibody (D-5) is recommended for detection of ACAD-8 of human origin by WB, IP, IF and ELISA
- Anti-ACAD-8 Antibody (D-5) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for ACAD-8 Antibody (D-5) for WB applications. These reagents are now offered in bundles with ACAD-8 Antibody (D-5) (see ordering information below).
ACAD-8 Antibody (D-5) is a mouse monoclonal IgG1 kappa light chain antibody that detects ACAD-8 of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). ACAD-8 (D-5) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. ACAD-8, also known as Isobutyryl-CoA dehydrogenase (IBD) or activator-recruited cofactor 42 kDa component (ARC42), plays a crucial role in fatty acid and amino acid catabolism, providing essential energy for heart and skeletal muscle. Structurally, ACAD-8 consists of an N-terminal α-helical domain, a central β-sheet domain, and a C-terminal α-helical domain, which are vital for enzymatic function. This mitochondrial flavoprotein specifically participates in valine degradation, converting isobutyryl-CoA to methacrylyl-CoA, and localizes to mitochondrial matrix where ACAD-8 forms a homotetramer. ACAD-8 deficiencies can lead to serious health issues, including carnitine deficiency, dilated cardiomyopathy, and feeding intolerance in infants, with isobutyryl-glycine excretion in urine serving as a diagnostic marker for ACAD-8 related defects.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
ACAD-8 Antibody (D-5) References:
- Composite co-activator ARC mediates chromatin-directed transcriptional activation. | Näär, AM., et al. 1999. Nature. 398: 828-32. PMID: 10235267
- Identification of isobutyryl-CoA dehydrogenase and its deficiency in humans. | Nguyen, TV., et al. 2002. Mol Genet Metab. 77: 68-79. PMID: 12359132
- Cloning and functional characterization of ACAD-9, a novel member of human acyl-CoA dehydrogenase family. | Zhang, J., et al. 2002. Biochem Biophys Res Commun. 297: 1033-42. PMID: 12359260
- Structures of isobutyryl-CoA dehydrogenase and enzyme-product complex: comparison with isovaleryl- and short-chain acyl-CoA dehydrogenases. | Battaile, KP., et al. 2004. J Biol Chem. 279: 16526-34. PMID: 14752098
- Isobutyryl-CoA dehydrogenase deficiency: isobutyrylglycinuria and ACAD8 gene mutations in two infants. | Sass, JO., et al. 2004. J Inherit Metab Dis. 27: 741-5. PMID: 15505379
- Convergent evolution of a 2-methylbutyryl-CoA dehydrogenase from isovaleryl-CoA dehydrogenase in Solanum tuberosum. | Goetzman, ES., et al. 2005. J Biol Chem. 280: 4873-9. PMID: 15574432
- Variations in IBD (ACAD8) in children with elevated C4-carnitine detected by tandem mass spectrometry newborn screening. | Pedersen, CB., et al. 2006. Pediatr Res. 60: 315-20. PMID: 16857760
- Development of a newborn screening follow-up algorithm for the diagnosis of isobutyryl-CoA dehydrogenase deficiency. | Oglesbee, D., et al. 2007. Genet Med. 9: 108-16. PMID: 17304052
- Isolated isobutyryl-CoA dehydrogenase deficiency: an unrecognized defect in human valine metabolism. | Roe, CR., et al. 1998. Mol Genet Metab. 65: 264-71. PMID: 9889013
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ACAD-8 Antibody (D-5) | sc-390038 | 200 µg/ml | $322.00 | |||
ACAD-8 Antibody (D-5): m-IgG Fc BP-HRP Bundle | sc-530048 | 200 µg Ab; 10 µg BP | $361.00 | |||
ACAD-8 Antibody (D-5): m-IgGκ BP-HRP Bundle | sc-523379 | 200 µg Ab, 40 µg BP | $361.00 | |||
ACAD-8 Antibody (D-5): m-IgG1 BP-HRP Bundle | sc-543839 | 200 µg Ab; 20 µg BP | $361.00 | |||
ACAD-8 Antibody (D-5) AC | sc-390038 AC | 500 µg/ml, 25% agarose | $424.00 | |||
ACAD-8 Antibody (D-5) HRP | sc-390038 HRP | 200 µg/ml | $322.00 | |||
ACAD-8 Antibody (D-5) FITC | sc-390038 FITC | 200 µg/ml | $336.00 | |||
ACAD-8 Antibody (D-5) PE | sc-390038 PE | 200 µg/ml | $349.00 | |||
ACAD-8 Antibody (D-5) Alexa Fluor® 488 | sc-390038 AF488 | 200 µg/ml | $364.00 | |||
ACAD-8 Antibody (D-5) Alexa Fluor® 546 | sc-390038 AF546 | 200 µg/ml | $364.00 | |||
ACAD-8 Antibody (D-5) Alexa Fluor® 594 | sc-390038 AF594 | 200 µg/ml | $364.00 | |||
ACAD-8 Antibody (D-5) Alexa Fluor® 647 | sc-390038 AF647 | 200 µg/ml | $364.00 | |||
ACAD-8 Antibody (D-5) Alexa Fluor® 680 | sc-390038 AF680 | 200 µg/ml | $364.00 | |||
ACAD-8 Antibody (D-5) Alexa Fluor® 790 | sc-390038 AF790 | 200 µg/ml | $364.00 |