Ac-lysine Antibody (AKL5C1): sc-32268
- Ac-lysine Antibody (AKL5C1) is a mouse monoclonal IgG1 κ Ac-lysine antibody, cited in 67 publications, provided at 200 µg/ml
- raised against chemically acetylated keyhole limpet hemocyanin
- Ac-lysine Antibody (AKL5C1) is recommended for detection of proteins containing N-epsilon-acetylated lysine residues by WB, IP, IF and IHC(P); non cross-reactive with N-α-acetylated lysine
- Ac-lysine Antibody (AKL5C1) is available conjugated to agarose for IP
- also available conjugated to Alexa Fluor® 594 for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for Ac-lysine Antibody (AKL5C1) for WB and IHC(P) applications. These reagents are now offered in bundles with Ac-lysine Antibody (AKL5C1) (see ordering information below).
QUICK LINKS
SEE ALSO...
Ac-lysine Antibody (AKL5C1) is a mouse monoclonal IgG1 kappa light chain antibody that detects Ac-lysine protein across various species through western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry with paraffin-embedded sections (IHCP). Anti-Ac-lysine antibody (AKL5C1) is available in non-conjugated and multiple conjugated forms, including agarose and various Alexa Fluor® conjugates, providing versatility for different experimental needs. Lysine acetylation, the modification recognized by Ac-lysine (AKL5C1) antibody, plays a crucial role in regulating gene expression and protein function, occurring in core histones, transcription factors, and numerous other proteins. This reversible modification is regulated by the dynamic interplay between acetyltransferases and deacetylases, which respond to cellular signals. Acetyl-lysine residues create specific docking sites for bromodomain-containing proteins, such as Gcn5, PCAF, TAF1, and CBP, which are essential for recruiting transcriptional co-activators and modulating chromatin structure. Histone acetylation, particularly N-terminal tails of histone H3, can be influenced by inhibitors like Trichostatin A, which enhances acetylation levels, and by external factors such as ethanol, which increases acetylation at specific lysine residues in a dose-dependent manner. Ac-lysine (AKL5C1) monoclonal antibody serves as an invaluable tool for studying gene regulation mechanisms and lysine acetylation′s functional implications in various biological contexts.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Ac-lysine Antibody (AKL5C1) References:
- Expanded lysine acetylation specificity of Gcn5 in native complexes. | Grant, PA., et al. 1999. J Biol Chem. 274: 5895-900. PMID: 10026213
- Phosphorylation of serine 10 in histone H3 is functionally linked in vitro and in vivo to Gcn5-mediated acetylation at lysine 14. | Lo, WS., et al. 2000. Mol Cell. 5: 917-26. PMID: 10911986
- Acetylation of histone H3 at lysine 9 by ethanol in rat hepatocytes. | Park, PH., et al. 2003. Biochem Biophys Res Commun. 306: 501-4. PMID: 12804592
- Phosphorylation at serine 28 and acetylation at lysine 9 of histone H3 induced by trichostatin A. | Zhong, S., et al. 2003. Oncogene. 22: 5291-7. PMID: 12917630
- Induction of PIG3 and NOXA through acetylation of p53 at 320 and 373 lysine residues as a mechanism for apoptotic cell death by histone deacetylase inhibitors. | Terui, T., et al. 2003. Cancer Res. 63: 8948-54. PMID: 14695212
- AML1 is functionally regulated through p300-mediated acetylation on specific lysine residues. | Yamaguchi, Y., et al. 2004. J Biol Chem. 279: 15630-8. PMID: 14752096
- Lysine acetylation and the bromodomain: a new partnership for signaling. | Yang, XJ. 2004. Bioessays. 26: 1076-87. PMID: 15382140
- Stat3 dimerization regulated by reversible acetylation of a single lysine residue. | Yuan, ZL., et al. 2005. Science. 307: 269-73. PMID: 15653507
- Structure and acetyl-lysine recognition of the bromodomain. | Mujtaba, S., et al. 2007. Oncogene. 26: 5521-7. PMID: 17694091
- The cardiac acetyl-lysine proteome. | Foster, DB., et al. 2013. PLoS One. 8: e67513. PMID: 23844019
- MYC acetylated lysine residues drive oncogenic cell transformation and regulate select genetic programs for cell adhesion-independent growth and survival. | Hurd, M., et al. 2023. Genes Dev. 37: 865-882. PMID: 37852796
- Acetylation of lysine 40 in alpha-tubulin is not essential in Tetrahymena thermophila. | Gaertig, J., et al. 1995. J Cell Biol. 129: 1301-10. PMID: 7775576
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Ac-lysine Antibody (AKL5C1) | sc-32268 | 200 µg/ml | $322.00 | |||
Ac-lysine Antibody (AKL5C1): m-IgG Fc BP-HRP Bundle | sc-528450 | 200 µg Ab; 10 µg BP | $361.00 | |||
Ac-lysine Antibody (AKL5C1): m-IgGκ BP-HRP Bundle | sc-520830 | 200 µg Ab, 40 µg BP | $361.00 | |||
Ac-lysine Antibody (AKL5C1): m-IgG1 BP-HRP Bundle | sc-542937 | 200 µg Ab; 20 µg BP | $361.00 | |||
Ac-lysine Antibody (AKL5C1) AC | sc-32268 AC | 500 µg/ml, 25% agarose | $424.00 | |||
Ac-lysine Antibody (AKL5C1) Alexa Fluor® 546 | sc-32268 AF546 | 200 µg/ml | $364.00 | |||
Ac-lysine Antibody (AKL5C1) Alexa Fluor® 594 | sc-32268 AF594 | 200 µg/ml | $364.00 | |||
Ac-lysine Antibody (AKL5C1) Alexa Fluor® 680 | sc-32268 AF680 | 200 µg/ml | $364.00 | |||
Ac-lysine Antibody (AKL5C1) Alexa Fluor® 790 | sc-32268 AF790 | 200 µg/ml | $364.00 |