Ac-lysine Antibody (7F8): sc-81623
- Ac-lysine Antibody (7F8) is a mouse monoclonal IgG1 κ, cited in 15 publications, provided at 200 µg/ml
- raised against acetylated keyhole limpet hemocyanin
- Ac-lysine Antibody (7F8) is recommended for detection of proteins containing acetylated lysine residues by WBIP
- Anti-Ac-lysine Antibody (7F8) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also provided azide-free for binding of Protein G, sc-81623 L, 200 µg/0.1 ml
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for Ac-lysine Antibody (7F8) for WB applications. These reagents are now offered in bundles with Ac-lysine Antibody (7F8) (see ordering information below).
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SEE ALSO...
Ac-lysine Antibody (7F8) is a mouse monoclonal IgG1 kappa light chain antibody that detects acetylated lysine by western blotting (WB) and immunoprecipitation (IP). Ac-lysine Antibody (7F8) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates, providing versatility for different experimental needs. Acetylated lysine plays a crucial role in regulating protein function and gene expression, as this post-translational modification influences chromatin structure and dynamics. Lysine acetylation is particularly important in histones, where acetylation can lead to a more relaxed chromatin state, facilitating transcriptional activation. The reversible nature of lysine acetylation, mediated by acetyltransferases and deacetylases, allows cells to respond dynamically to various signals, making acetylation a vital mechanism in cellular regulation. Acetyl-lysine residues serve as specific docking sites for bromodomain-containing proteins, such as Gcn5, PCAF, TAF1, and CBP, which are essential for recruiting transcriptional machinery and modulating gene expression. Anti-acetylated lysine antibody (7F8) specifically recognizes acetylated lysine residues, making this antibody an invaluable tool for studying gene regulation and protein interactions.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Ac-lysine Antibody (7F8) References:
- Small molecule inhibitors of bromodomain-acetyl-lysine interactions. | Brand, M., et al. 2015. ACS Chem Biol. 10: 22-39. PMID: 25549280
- Acetyl-lysine erasers and readers in the control of pulmonary hypertension and right ventricular hypertrophy. | Stratton, MS. and McKinsey, TA. 2015. Biochem Cell Biol. 93: 149-57. PMID: 25707943
- Dual Genetic Encoding of Acetyl-lysine and Non-deacetylatable Thioacetyl-lysine Mediated by Flexizyme. | Xiong, H., et al. 2016. Angew Chem Int Ed Engl. 55: 4083-6. PMID: 26914285
- SPOTing Acetyl-Lysine Dependent Interactions. | Picaud, S. and Filippakopoulos, P. 2015. Microarrays (Basel). 4: 370-88. PMID: 27600229
- An Alternative Strategy for Pan-acetyl-lysine Antibody Generation. | Kim, SY., et al. 2016. PLoS One. 11: e0162528. PMID: 27606599
- Site-Specific Acetyl Lysine Antibodies Reveal Differential Regulation of Histone Acetylation upon Kinase Inhibition. | Chen, S., et al. 2017. Cell Biochem Biophys. 75: 119-129. PMID: 27990613
- Disrupting Acetyl-lysine Interactions: Recent Advance in the Development of BET Inhibitors. | Zhang, F. and Ma, S. 2018. Curr Drug Targets. 19: 1148-1165. PMID: 29189147
- Synthesis of Nε-acetyl-L-homolysine by the Lossen rearrangement and its application for probing deacetylases and binding modules of acetyl-lysine. | Rehkopf, L., et al. 2023. J Pept Sci. 29: e3462. PMID: 36416071
- Synthesis and anticancer evaluation of acetylated-lysine conjugated gemcitabine prodrugs. | Wang, M., et al. 2023. RSC Med Chem. 14: 1572-1580. PMID: 37593582
- Binding of Acetylated Lysine by Using a Water Soluble Aryl Extended Calix[4]pyrrole. | Orlandini, M., et al. 2024. Chemistry. 30: e202303715. PMID: 38158380
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Ac-lysine Antibody (7F8) | sc-81623 | 200 µg/ml | $316.00 | |||
Ac-lysine Antibody (7F8): m-IgG Fc BP-HRP Bundle | sc-528707 | 200 µg Ab; 10 µg BP | $354.00 | |||
Ac-lysine Antibody (7F8): m-IgGκ BP-HRP Bundle | sc-521175 | 200 µg Ab, 40 µg BP | $354.00 | |||
Ac-lysine Antibody (7F8): m-IgG1 BP-HRP Bundle | sc-543078 | 200 µg Ab; 20 µg BP | $354.00 | |||
Ac-lysine Antibody (7F8) L | sc-81623 L | 200 µg/0.1 ml | $316.00 | |||
Ac-lysine Antibody (7F8) AC | sc-81623 AC | 500 µg/ml, 25% agarose | $416.00 | |||
Ac-lysine Antibody (7F8) HRP | sc-81623 HRP | 200 µg/ml | $316.00 | |||
Ac-lysine Antibody (7F8) FITC | sc-81623 FITC | 200 µg/ml | $330.00 | |||
Ac-lysine Antibody (7F8) PE | sc-81623 PE | 200 µg/ml | $343.00 | |||
Ac-lysine Antibody (7F8) Alexa Fluor® 488 | sc-81623 AF488 | 200 µg/ml | $357.00 | |||
Ac-lysine Antibody (7F8) Alexa Fluor® 546 | sc-81623 AF546 | 200 µg/ml | $357.00 | |||
Ac-lysine Antibody (7F8) Alexa Fluor® 594 | sc-81623 AF594 | 200 µg/ml | $357.00 | |||
Ac-lysine Antibody (7F8) Alexa Fluor® 647 | sc-81623 AF647 | 200 µg/ml | $357.00 | |||
Ac-lysine Antibody (7F8) Alexa Fluor® 680 | sc-81623 AF680 | 200 µg/ml | $357.00 | |||
Ac-lysine Antibody (7F8) Alexa Fluor® 790 | sc-81623 AF790 | 200 µg/ml | $357.00 |