ABT1 Antibody (B-9): sc-390233
- ABT1 Antibody (B-9) is a mouse monoclonal IgG1 κ ABT1 antibody provided at 200 µg/ml
- raised against amino acids 136-237 mapping within an internal region of ABT1 of human origin
- ABT1 Antibody (B-9) is recommended for detection of ABT1 of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-ABT1 Antibody (B-9) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of ABT1 (B-9): sc-390233.
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for ABT1 Antibody (B-9) for WB applications. These reagents are now offered in bundles with ABT1 Antibody (B-9) (see ordering information below).
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SEE ALSO...
ABT1 Antibody (B-9) is a mouse monoclonal IgG1 kappa light chain antibody that detects ABT1 of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). ABT1 Antibody (B-9) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. ABT1, known as activator of basal transcription 1, plays a crucial role in gene expression regulation by associating with TATA-binding protein (TBP) to enhance basal transcription activity of class II promoters. This interaction facilitates transcription complex assembly necessary for gene activation in the nucleus. ABT1′s ability to enhance transcription is particularly important in cellular processes such as growth and differentiation, where precise gene regulation is essential. ABT1 associates with TBP in HeLa nuclear extracts, underscoring ABT1′s significance in transcriptional regulation. ABT1 also interacts with Ets2 Repressor Factor (ERF), a member of the ETS family of transcription factors, which modulates transcriptional activity and regulates cell proliferation and differentiation pathways.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
ABT1 Antibody (B-9) References:
- A novel TATA-binding protein-binding protein, ABT1, activates basal transcription and has a yeast homolog that is essential for growth. | Oda, T., et al. 2000. Mol Cell Biol. 20: 1407-18. PMID: 10648625
- ERF: an ETS domain protein with strong transcriptional repressor activity, can suppress ets-associated tumorigenesis and is regulated by phosphorylation during cell cycle and mitogenic stimulation. | Sgouras, DN., et al. 1995. EMBO J. 14: 4781-93. PMID: 7588608
- ERF: genomic organization, chromosomal localization and promoter analysis of the human and mouse genes. | Liu, D., et al. 1997. Oncogene. 14: 1445-51. PMID: 9136988
- Genomic structure and chromosomal localization of the novel ETS factor, PE-2 (ERF). | de Castro, CM., et al. 1997. Genomics. 42: 227-35. PMID: 9192842
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ABT1 Antibody (B-9) | sc-390233 | 200 µg/ml | $316.00 | |||
ABT1 Antibody (B-9): m-IgG Fc BP-HRP Bundle | sc-530101 | 200 µg Ab; 10 µg BP | $354.00 | |||
ABT1 Antibody (B-9): m-IgGκ BP-HRP Bundle | sc-523457 | 200 µg Ab, 40 µg BP | $354.00 | |||
ABT1 Antibody (B-9): m-IgG1 BP-HRP Bundle | sc-543871 | 200 µg Ab; 20 µg BP | $354.00 | |||
ABT1 Antibody (B-9) AC | sc-390233 AC | 500 µg/ml, 25% agarose | $416.00 | |||
ABT1 Antibody (B-9) HRP | sc-390233 HRP | 200 µg/ml | $316.00 | |||
ABT1 Antibody (B-9) FITC | sc-390233 FITC | 200 µg/ml | $330.00 | |||
ABT1 Antibody (B-9) PE | sc-390233 PE | 200 µg/ml | $343.00 | |||
ABT1 Antibody (B-9) Alexa Fluor® 488 | sc-390233 AF488 | 200 µg/ml | $357.00 | |||
ABT1 Antibody (B-9) Alexa Fluor® 546 | sc-390233 AF546 | 200 µg/ml | $357.00 | |||
ABT1 Antibody (B-9) Alexa Fluor® 594 | sc-390233 AF594 | 200 µg/ml | $357.00 | |||
ABT1 Antibody (B-9) Alexa Fluor® 647 | sc-390233 AF647 | 200 µg/ml | $357.00 | |||
ABT1 Antibody (B-9) Alexa Fluor® 680 | sc-390233 AF680 | 200 µg/ml | $357.00 | |||
ABT1 Antibody (B-9) Alexa Fluor® 790 | sc-390233 AF790 | 200 µg/ml | $357.00 |