AADAC Antibody (G-12): sc-390591

AADAC Antibody (G-12) is a mouse monoclonal IgG2b kappa light chain antibody that detects AADAC protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). AADAC (G-12) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. AADAC, or arylacetamide deacetylase, is a crucial enzyme located in the endoplasmic reticulum, primarily expressed in hepatocytes, intestinal mucosal cells, the pancreas, and the adrenal gland. This single-pass type II membrane protein plays a significant role in lipid metabolism, particularly in the assembly of very-low-density lipoproteins (VLDLs) by mobilizing triacylglycerol from the cytosolic pool through lipolysis and re-esterification. AADAC contributes to the metabolic activation of arylamine substrates, which can lead to the formation of ultimate carcinogens. AADAC interacts with related proteins such as AADACL1 and AADACL2, which are also involved in lipid metabolism and can influence cancer cell behavior. Disruption of AADAC function can impair ether lipid metabolism, affecting cell migration and tumor growth, highlighting its potential as a target for therapeutic intervention in cancer and metabolic disorders.

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AADAC Antibody (G-12) References:

1. Characterization of the rodent genes for arylacetamide deacetylase, a putative microsomal lipase, and evidence for transcriptional regulation.  |  Trickett, JI., et al. 2001. J Biol Chem. 276: 39522-32. PMID: 11481320
2. Synthesis and function of hepatic very-low-density lipoprotein.  |  Gibbons, GF., et al. 2004. Biochem Soc Trans. 32: 59-64. PMID: 14748713
3. An enzyme that regulates ether lipid signaling pathways in cancer annotated by multidimensional profiling.  |  Chiang, KP., et al. 2006. Chem Biol. 13: 1041-50. PMID: 17052608
4. Purification and characterization of a human liver arylacetamide deacetylase.  |  Probst, MR., et al. 1991. Biochem Biophys Res Commun. 177: 453-9. PMID: 2043131
5. Human liver arylacetamide deacetylase. Molecular cloning of a novel esterase involved in the metabolic activation of arylamine carcinogens with high sequence similarity to hormone-sensitive lipase.  |  Probst, MR., et al. 1994. J Biol Chem. 269: 21650-6. PMID: 8063807
6. Radiation hybrid mapping of human arylacetamide deacetylase (AADAC) locus to chromosome 3.  |  Yamazaki, K., et al. 1997. Genomics. 44: 248-50. PMID: 9299245

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-AADAC Antibody (G-12) has 3 citations in a variety of scientific publications.

Primary antibodies like Anti-AADAC Antibody (G-12) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

AADAC Antibody (G-12) Specifications

Alternate Product Name: AADAC

Product Clone Name: G-12

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 289-311

Epitope Target: AADAC

Epitope Species: human

Product Reactivity/Detection: AADAC

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 3 citations in various scientific publications

Available Conjugates/Forms for AADAC Antibody (G-12): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin

The preferred AADAC antibody is AADAC Antibody (H-8): sc-390592.