A20/TNFAIP3 Antibody (B-5): sc-376564

A20 Antibody (B-5) is a mouse monoclonal IgG1 antibody that detects A20 in human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). A20 is a Cys2/Cys2 zinc finger protein that plays a pivotal role in regulating inflammatory responses by acting as a negative regulator of nuclear factor κ B (NFκB) gene expression. This regulation is crucial because NFκB is a key transcription factor involved in the immune response, and uncontrolled activation can lead to chronic inflammation and various diseases. A20 is induced by inflammatory stimuli such as tumor necrosis factor (TNF) and interleukin 1 (IL-1), effectively terminating NFκB responses by inhibiting activation. A20′s structure includes seven zinc finger domains, with four being essential for in vitro inhibition of TNF-induced NFκB activation. A20 interacts with several proteins, including TRAF2, TRAF6, and IκB kinase (IKK) γ, which further enhances ability to prevent cell death. TXBP151, a novel A20-binding protein, may play a role in mediating A20′s anti-apoptotic activity. Given involvement in the negative feedback regulation of signal transduction, A20 and binding partners present promising avenues for therapeutic intervention in a range of inflammatory diseases.

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A20/TNFAIP3 Antibody (B-5) References:

1. Transgenic expression of A20 prevents cardiac cell death and myocardial dysfunction after myocardial infarction.  |  Shaw, J., et al. 2007. Circulation. 115: 1827-9. PMID: 17420361
2. Genetic relationships between A20/TNFAIP3, chronic inflammation and autoimmune disease.  |  Vereecke, L., et al. 2011. Biochem Soc Trans. 39: 1086-91. PMID: 21787353
3. A20 inhibits lipopolysaccharide-induced inflammation in enterocytes.  |  Zheng, CF., et al. 2016. World J Gastrointest Pharmacol Ther. 7: 540-549. PMID: 27867687
4. Emerging role of zinc finger protein A20 as a suppressor of hepatocellular carcinoma.  |  Yi, PS., et al. 2019. J Cell Physiol. 234: 21479-21484. PMID: 31134613
5. A20 functions as a negative regulator in macrophage for DSS-induced colitis.  |  Pu, T., et al. 2021. Int Immunopharmacol. 97: 107804. PMID: 34062371
6. A20 Establishes Negative Feedback With TRAF6/NF-κB and Attenuates Early Brain Injury After Experimental Subarachnoid Hemorrhage.  |  Deng, HJ., et al. 2021. Front Immunol. 12: 623256. PMID: 34381441
7. A20 controls expression of beta-cell regulatory genes and transcription factors.  |  Ratajczak, W., et al. 2021. J Mol Endocrinol. 67: 189-201. PMID: 34463643
8. Th17 Cells in Periodontitis and Its Regulation by A20.  |  Huang, N., et al. 2021. Front Immunol. 12: 742925. PMID: 34557201
9. MALT1 promotes necroptosis in stroke rat brain via targeting the A20/RIPK3 pathway.  |  Peng, ZM., et al. 2023. Arch Biochem Biophys. 735: 109502. PMID: 36603698
10. Zinc attenuates monocrotaline-induced pulmonary hypertension in rats through upregulation of A20.  |  Chen, W., et al. 2024. J Mol Cell Cardiol. 195: 24-35. PMID: 39002608

The preferred A20/TNFAIP3 antibody is A20/TNFAIP3 Antibody (A-12): sc-166692.