8-OHdG Antibody (E-8): sc-393871

8-OHdG Antibody (E-8) is a mouse monoclonal IgM kappa antibody that detects 8-OHdG protein of non-species reactive by immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). anti-8-OHdG antibody (E-8) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates, providing versatility for different experimental needs. 8-OHdG (8-hydroxy-2′-deoxyguanosine) serves as a critical biomarker for oxidative DNA damage, which can significantly impair cellular function and increase the risk of tumorigenesis. 8-OHdG is generated through the oxidation of guanine residues in DNA, primarily by reactive oxygen and nitrogen species such as hydroxyl radicals and peroxynitrite. 8-OHdG presence in cells indicates oxidative stress and has been linked to various diseases, including cancer, neurodegenerative disorders, and cardiovascular diseases. 8-OHdG monoclonal antibody (E-8) helps researchers study DNA damage and repair mechanisms, as well as oxidative stress in disease progression, making this tool valuable for molecular biology and cancer research.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

8-OHdG Antibody (E-8) References:

1. 8OHdG is not a biomarker for Huntington disease state or progression.  |  Borowsky, B., et al. 2013. Neurology. 80: 1934-41. PMID: 23616162
2. Automated Cell-Based Quantitation of 8-OHdG Damage.  |  Debelec-Butuner, B., et al. 2016. Methods Mol Biol. 1516: 299-308. PMID: 27044043
3. Increased Plasma Levels of 8-Hydroxy-2'-deoxyguanosine (8-OHdG) in Patients with Pseudoexfoliation Glaucoma.  |  Kondkar, AA., et al. 2019. J Ophthalmol. 2019: 8319563. PMID: 31341657
4. Elevated cadmium and 8-hydroxy-2'-deoxyguanosine (8-OHdG) levels in residents living near electroplating industries.  |  Xu, P., et al. 2021. Environ Sci Pollut Res Int. 28: 34427-34435. PMID: 33651290
5. 8-Hydroxy-2'-deoxyguanosine (8-OHdG) as a biomarker of oxidative DNA damage induced by occupational exposure to nanomaterials: a systematic review.  |  Omari Shekaftik, S. and Nasirzadeh, N. 2021. Nanotoxicology. 15: 850-864. PMID: 34171202
6. Rapid extraction and analysis of oxidative stress and DNA damage biomarker 8-hydroxy-2'-deoxyguanosine (8-OHdG) in urine: Application to a study with pregnant women.  |  Bláhová, L., et al. 2023. Int J Hyg Environ Health. 250: 114175. PMID: 37105016
7. Dietary total antioxidant capacity and serum 8-OHdG in patients with schizophrenia.  |  Karagöl, G., et al. 2023. Int J Psychiatry Med. 58: 544-558. PMID: 37210638
8. Enhancing Angioinvasion Assessment in Papillary Thyroid Cancer Via a Biomarker Panel Involving TAC, 8-OHdG, and Sortilin.  |  Buczyńska, A., et al. 2024. J Clin Endocrinol Metab. 109: 1866-1872. PMID: 38181425
9. Comparative evaluation of salivary, serum and urinary 8-OHdG in gutka-associated oral submucous fibrosis.  |  Prajapati, RK., et al. 2024. J Oral Maxillofac Pathol. 28: 37-41. PMID: 38800434
10. Urinary 8-hydroxy-2'-deoxyguanosine (8-OHdG) levels in women with or without gynecologic cancer.  |  Yamamoto, T., et al. 1996. J Obstet Gynaecol Res. 22: 359-63. PMID: 8870419

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-8-OHdG Antibody (E-8) has 77 citations in a variety of scientific publications.

Primary antibodies for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

8-OHdG Antibody (E-8) Specifications

Alternate Product Name: 8-OHdG

Product Clone Name: E-8

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgM kappa light chain

Amino Acids:

Epitope Target: 8-OHdG

Epitope Species: synthetic

Product Reactivity/Detection: 8-OHdG (8-Hydroxy-2′-deoxyguanosine)

Species Reactivity/Detection: NSR

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgM kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 77 citations in various scientific publications

Available Conjugates/Forms for 8-OHdG Antibody (E-8): AC: Agarose, AF488: Alexa Fluor^® 488, AF647: Alexa Fluor^® 647, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred 8-OHdG antibody is 8-OHdG Antibody (15A3): sc-66036.