8-OHdG Antibody (15A3): sc-66036

8-OHdG Antibody (15A3) is a mouse monoclonal IgG2b antibody that detects 8-OHdG (8-hydroxy-2′-deoxyguanosine, 8-hydroxyguanine, and 8-hydroxyguanosine) in non-species reactive samples through immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). 8-OHdG is a critical biomarker for oxidative DNA damage, which can significantly impair cellular function and increase the risk of tumorigenesis. The presence of 8-hydroxy-2′-deoxyguanosine in cells indicates oxidative stress, as reactive oxygen and nitrogen species, including hydroxyl radicals and peroxynitrite, generate this modification. 8-hydroxyguanine causes G to T transversions, a mutation type that can contribute to cancer development. Anti-8-OHdG antibody (15A3) enables researchers to study oxidative damage implications on DNA and RNA, providing valuable insights into mutagenesis mechanisms and cellular responses to oxidative stress. 8-OHdG (15A3) antibody is available in a non-conjugated form, making this reagent versatile for various experimental setups.

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8-OHdG Antibody (15A3) References:

1. Alteration of 8-hydroxyguanosine concentrations in the cerebrospinal fluid and serum from patients with Parkinson's disease.  |  Abe, T., et al. 2003. Neurosci Lett. 336: 105-8. PMID: 12499051
2. 8-Hydroxyguanosine repair is defective in some microsatellite stable colorectal cancer cells.  |  Parker, AR., et al. 2002. Cancer Res. 62: 7230-3. PMID: 12499263
3. Normal somatic hypermutation of Ig genes in the absence of 8-hydroxyguanine-DNA glycosylase.  |  Winter, DB., et al. 2003. J Immunol. 170: 5558-62. PMID: 12759433
4. Accumulation of the oxidative base lesion 8-hydroxyguanine in DNA of tumor-prone mice defective in both the Myh and Ogg1 DNA glycosylases.  |  Russo, MT., et al. 2004. Cancer Res. 64: 4411-4. PMID: 15231648
5. Significance of 8-hydroxy-2'-deoxyguanosine levels in patients with idiopathic dilated cardiomyopathy.  |  Watanabe, E., et al. 2006. J Card Fail. 12: 527-32. PMID: 16952786
6. UVA-induced degradation of 8-hydroxyguanine in oligonucleotide and the effect on its activities in yeast oligonucleotide transformation assay.  |  Okugawa, Y., et al. 2004. Nucleic Acids Symp Ser (Oxf). 287-8. PMID: 17150591
7. The role of metal ion binding in generating 8-hydroxy-2'-deoxyguanosine from the nucleoside 2'-deoxyguanosine and the nucleotide 2'-deoxyguanosine-5'-monophosphate.  |  Noblitt, SD., et al. 2007. J Inorg Biochem. 101: 536-42. PMID: 17234269
8. Urinary 8-hydroxy-2'-deoxyguanosine (8-OHdG) and genetic polymorphisms in breast cancer patients.  |  Kuo, HW., et al. 2007. Mutat Res. 631: 62-8. PMID: 17512776
9. 8-Oxo-7,8-Dihydro-2'-Deoxyguanosine (8-oxodG) and 8-Hydroxy-2'-Deoxyguanosine (8-OHdG) as a Potential Biomarker for Gestational Diabetes Mellitus (GDM) Development.  |  Urbaniak, SK., et al. 2020. Molecules. 25: PMID: 31947819
10. CypD induced ROS output promotes intracranial aneurysm formation and rupture by 8-OHdG/NLRP3/MMP9 pathway.  |  Fan, H., et al. 2023. Redox Biol. 67: 102887. PMID: 37717465
11. Using 8-Hydroxy-2'-Deoxiguanosine (8-OHdG) as a Reliable Biomarker for Assessing Periodontal Disease Associated with Diabetes.  |  Goriuc, A., et al. 2024. Int J Mol Sci. 25: PMID: 38338704
12. Prognostic and aetiological relevance of 8-hydroxyguanosine in human breast carcinogenesis.  |  Musarrat, J., et al. 1996. Eur J Cancer. 32A: 1209-14. PMID: 8758255

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-8-OHdG Antibody (15A3) has 222 citations in a variety of scientific publications.

Primary antibodies for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

8-OHdG Antibody (15A3) Specifications

Alternate Product Name: 8-OHdG

Product Clone Name: 15A3

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b

Amino Acids:

Epitope Target: 8-OHdG

Epitope Species:

Product Reactivity/Detection: 8-OHdG (8-hydroxy-2'-deoxyguanosine, 8-hydroxyguanine and 8-hydroxyguanosine)

Species Reactivity/Detection: NSR

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: IF, IHC(P), ELISA

Vialing Information: Each vial contains 100 µg IgG_2b in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 222 citations in various scientific publications

Available Conjugates/Forms for 8-OHdG Antibody (15A3): N/A