53BP1 Antibody (6B3E10): sc-517281
- 53BP1 Antibody (6B3E10) is a mouse monoclonal IgG1, cited in 9 publications, provided at 200 µg/ml
- raised against amino acids 574-773 of 53BP1 of human origin
- recommended for detection of 53BP1 of human origin by WB, IP, IF, IHC(P), FCM and ELISA
- m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for 53BP1 Antibody (6B3E10) for WB and IHC(P) applications. These reagents are now offered in bundles with 53BP1 Antibody (6B3E10) (see ordering information below).
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53BP1 Antibody (6B3E10) is a mouse monoclonal IgG1 antibody that detects 53BP1 in human samples through various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), flow cytometry (FCM), and enzyme-linked immunosorbent assay (ELISA). 53BP1 plays a crucial role in the cellular response to DNA damage, particularly in double-strand breaks, by facilitating repair processes and maintaining genomic stability. Anti-53BP1 antibody (6B3E10) recognizes nuclear localization, where 53BP1 is essential for activating DNA damage response pathways and managing DNA lesions. 53BP1 dysregulation can lead to genomic instability and contribute to tumorigenesis. Furthermore, 53BP1 interacts with various proteins involved in DNA repair, including p53, highlighting a significant role in tumor suppression mechanisms. Anti-53BP1 antibody (6B3E10) serves as a valuable tool for studying DNA damage response pathways and their implications in cancer biology.
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53BP1 Antibody (6B3E10) References:
- 53BP1 Enforces Distinct Pre- and Post-resection Blocks on Homologous Recombination. | Callen, E., et al. 2020. Mol Cell. 77: 26-38.e7. PMID: 31653568
- ATM-phosphorylated SPOP contributes to 53BP1 exclusion from chromatin during DNA replication. | Wang, D., et al. 2021. Sci Adv. 7: PMID: 34144977
- 53BP1 regulates heterochromatin through liquid phase separation. | Zhang, L., et al. 2022. Nat Commun. 13: 360. PMID: 35042897
- Multifaceted regulation and functions of 53BP1 in NHEJ‑mediated DSB repair (Review). | Lei, T., et al. 2022. Int J Mol Med. 50: PMID: 35583003
- HDGFRP3 interaction with 53BP1 promotes DNA double-strand break repair. | Zhang, Z., et al. 2023. Nucleic Acids Res. 51: 2238-2256. PMID: 36794849
- ATM-ESCO2-SMC3 axis promotes 53BP1 recruitment in response to DNA damage and safeguards genome integrity by stabilizing cohesin complex. | Fu, J., et al. 2023. Nucleic Acids Res. 51: 7376-7391. PMID: 37377435
- DNA repair and antibody diversification: the 53BP1 paradigm. | Kabrani, E., et al. 2023. Trends Immunol. 44: 782-791. PMID: 37640588
- 53BP1 interacts with the RNA primer from Okazaki fragments to support their processing during unperturbed DNA replication. | Leriche, M., et al. 2023. Cell Rep. 42: 113412. PMID: 37963016
- 53BP1-mediated activation of the tumor suppressor p53. | Belal, H., et al. 2024. Curr Opin Cell Biol. 91: 102424. PMID: 39244835
- 53BP1 deficiency leads to hyperrecombination using break-induced replication (BIR). | Shah, SB., et al. 2024. Nat Commun. 15: 8648. PMID: 39368985
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
53BP1 Antibody (6B3E10) | sc-517281 | 200 µg/ml | $322.00 | |||
53BP1 Antibody (6B3E10): m-IgG Fc BP-HRP Bundle | sc-541101 | 200 µg Ab; 10 µg BP | $361.00 | |||
53BP1 Antibody (6B3E10): m-IgG1 BP-HRP Bundle | sc-542580 | 200 µg Ab; 20 µg BP | $361.00 |