5-LO Antibody (33): sc-136195

5-LO Antibody (33) is a mouse monoclonal IgG1 kappa light chain antibody that detects 5-LO in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), and immunofluorescence (IF). Anti-5-LO antibody (33) is available as a non-conjugated format. 5-lipoxygenase (5-LO) plays a crucial role in the biosynthesis of leukotrienes, which are lipid mediators involved in inflammatory responses, particularly in conditions such as asthma and allergic reactions. 5-LO catalyzes the initial steps in the conversion of arachidonic acid into leukotrienes, which induce bronchoconstriction and increase vascular permeability, contributing to the pathophysiology of various inflammatory diseases. 5-LO localization within cells varies depending on cell type and activation state. In activated polymorphonuclear leukocytes, 5-LO translocates from the cytosol to the nuclear envelope in a calcium-dependent manner, while in alveolar macrophages, 5-LO predominantly resides in the nucleus. Upon activation, intranuclear 5-LO associates with the nuclear membrane, a process influenced by interactions with specific signaling molecules, phosphorylation events, and a unique nuclear localization signal encoded at the amino terminus. Understanding 5-LO dynamic localization and function is essential for elucidating inflammatory processes and developing targeted therapies for related diseases.

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5-LO Antibody (33) References:

1. Identification of a bipartite nuclear localization sequence necessary for nuclear import of 5-lipoxygenase.  |  Healy, AM., et al. 1999. J Biol Chem. 274: 29812-8. PMID: 10514459
2. 5-lipoxygenase mRNA and protein isoforms.  |  Ochs, MJ., et al. 2014. Basic Clin Pharmacol Toxicol. 114: 78-82. PMID: 24020397
3. Molecular cloning and amino acid sequence of human 5-lipoxygenase.  |  Matsumoto, T., et al. 1988. Proc Natl Acad Sci U S A. 85: 26-30. PMID: 2829172
4. Inhibition of 5-Lipoxygenase (5-Lo) Attenuates Inflammation and Bone Resorption in Lipopolysaccharide (Lps)-Induced Periodontal Disease.  |  Lopes, DEM., et al. 2017. J Periodontol. 1-18. PMID: 28871891
5. Modulation of microRNA processing by 5-lipoxygenase.  |  Uebbing, S., et al. 2021. FASEB J. 35: e21193. PMID: 33205517
6. 5-Lipoxygenase as an emerging target against age-related brain disorders.  |  Yan, M., et al. 2021. Ageing Res Rev. 69: 101359. PMID: 33984528
7. The role of leukotriene inhibition using a 5-lipoxygenase (5-LO) inhibitor in a joint contracture model.  |  Jeffs, AD., et al. 2023. J Exp Orthop. 10: 64. PMID: 37341811
8. 5-Lipoxygenase is located in the euchromatin of the nucleus in resting human alveolar macrophages and translocates to the nuclear envelope upon cell activation.  |  Woods, JW., et al. 1995. J Clin Invest. 95: 2035-46. PMID: 7738170
9. Influence of arachidonic acid on indices of phospholipase A2 activity in the human neutrophil.  |  Winkler, JD., et al. 1993. Biochem J. 291 (Pt 3): 825-31. PMID: 8387780
10. Tyrosine kinase activity modulates catalysis and translocation of cellular 5-lipoxygenase.  |  Lepley, RA., et al. 1996. J Biol Chem. 271: 6179-84. PMID: 8626407
11. Colocalization of cytosolic phospholipase A2, 5-lipoxygenase, and 5-lipoxygenase-activating protein at the nuclear membrane of A23187-stimulated human neutrophils.  |  Pouliot, M., et al. 1996. Eur J Biochem. 238: 250-8. PMID: 8665944
12. Determinants of 5-lipoxygenase nuclear localization using green fluorescent protein/5-lipoxygenase fusion proteins.  |  Chen, XS., et al. 1998. J Biol Chem. 273: 31237-44. PMID: 9813031

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-5-LO Antibody (33) has 18 citations in a variety of scientific publications.

Primary antibodies like Anti-5-LO Antibody (33) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

5-LO Antibody (33) Specifications

Alternate Product Name: ALOX5

Product Clone Name: 33

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 442-590

Epitope Target: 5-LO

Epitope Species: human

Product Reactivity/Detection: 5-LO

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: Arabadopsis

Applications: WB, IP, IF

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 18 citations in various scientific publications

Available Conjugates/Forms for 5-LO Antibody (33): N/A

The preferred 5-LO antibody is 5-LO Antibody (B-3): sc-518174.