5-LO Antibody (B-3): sc-518174
- 5-LO Antibody (B-3) is a mouse monoclonal IgG2a κ provided at 200 µg/ml
- raised against amino acids 106-225 mapping near the N-terminus of 5-LO of human origin
- recommended for detection of 5-LO of human origin by WB, IP, IF, IHC(P) and ELISA
- Anti-5-LO Antibody (B-3) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgGκ BP-HRP is the preferred secondary detection reagent for 5-LO Antibody (B-3) for WB and IHC(P) applications. This reagent is now offered in a bundle with 5-LO Antibody (B-3) (see ordering information below). For additional m-IgGκ BP conjugates see our complete list of Mouse IgG Binding Proteins.
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5-LO Antibody (B-3) is a mouse monoclonal IgG2a kappa light chain antibody that detects 5-lipoxygenase (5-LO) of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-5-LO antibody (B-3) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. 5-lipoxygenase plays a crucial role in inflammatory response by catalyzing the first two steps in leukotriene biosynthesis, which are lipid mediators contributing to bronchoconstriction and increased vascular permeability during allergic reactions and asthma. 5-LO is predominantly expressed in polymorphonuclear leukocytes, macrophages, and mast cells, with cellular localization being particularly significant. In activated blood polymorphonuclear leukocytes, 5-LO translocates from cytosol to nuclear envelope in a calcium-dependent manner, while in alveolar macrophages, 5-LO is primarily localized in the nucleus. Upon activation, intranuclear 5-LO associates with nuclear membrane, a process influenced by various signaling molecules, phosphorylation events, and a specific nuclear localization signal encoded at the amino terminus. This dynamic intracellular shuttling of 5-LO is essential for leukotriene synthesis and highlights the importance of precise localization in regulating inflammatory responses.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
5-LO Antibody (B-3) References:
- Therapeutic options for 5-lipoxygenase inhibitors. | Werz, O. and Steinhilber, D. 2006. Pharmacol Ther. 112: 701-18. PMID: 16837050
- The role of 5-lipoxygenase and leukotrienes in shock and ischemia-reperfusion injury. | Rossi, A., et al. 2007. ScientificWorldJournal. 7: 56-74. PMID: 17334599
- Cyclooxygenases and 5-lipoxygenase in Alzheimer's disease. | Manev, H., et al. 2011. Prog Neuropsychopharmacol Biol Psychiatry. 35: 315-9. PMID: 20691748
- Novel 5-lipoxygenase isoforms affect the biosynthesis of 5-lipoxygenase products. | Boudreau, LH., et al. 2011. FASEB J. 25: 1097-105. PMID: 21098726
- Effect of a 5-lipoxygenase (5-LO)/cyclooxygenase (CO) inhibitor, WY-47, 288, on cutaneous models of inflammation. | Carlson, RP., et al. 1989. Agents Actions. 26: 319-28. PMID: 2500009
- 5-Lipoxygenase, a key enzyme for leukotriene biosynthesis in health and disease. | Rådmark, O., et al. 2015. Biochim Biophys Acta. 1851: 331-9. PMID: 25152163
- Inhibition of 5-lipoxygenase alleviates graft-versus-host disease. | Rezende, BM., et al. 2017. J Exp Med. 214: 3399-3415. PMID: 28947611
- The inhibition of 5-Lipoxygenase (5-LO) products leukotriene B4 (LTB4) and cysteinyl leukotrienes (cysLTs) modulates the inflammatory response and improves cutaneous wound healing. | Guimarães, FR., et al. 2018. Clin Immunol. 190: 74-83. PMID: 28965882
- Human 5-lipoxygenase regulates transcription by association to euchromatin. | Kreiß, M., et al. 2022. Biochem Pharmacol. 203: 115187. PMID: 35878796
- Synovial 5-Lipoxygenase-Derived Oxylipins Define a Lympho-Myeloid-Enriched Synovium. | Murillo-Saich, JD., et al. 2024. Arthritis Rheumatol. 76: 1230-1242. PMID: 38508862
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
5-LO Antibody (B-3) | sc-518174 | 200 µg/ml | $316.00 | |||
5-LO Antibody (B-3): m-IgGκ BP-HRP Bundle | sc-550698 | 200 µg Ab; 40 µg BP | $354.00 | |||
5-LO Antibody (B-3) AC | sc-518174 AC | 500 µg/ml, 25% agarose | $416.00 | |||
5-LO Antibody (B-3) HRP | sc-518174 HRP | 200 µg/ml | $316.00 | |||
5-LO Antibody (B-3) FITC | sc-518174 FITC | 200 µg/ml | $330.00 | |||
5-LO Antibody (B-3) PE | sc-518174 PE | 200 µg/ml | $343.00 | |||
5-LO Antibody (B-3) Alexa Fluor® 488 | sc-518174 AF488 | 200 µg/ml | $357.00 | |||
5-LO Antibody (B-3) Alexa Fluor® 546 | sc-518174 AF546 | 200 µg/ml | $357.00 | |||
5-LO Antibody (B-3) Alexa Fluor® 594 | sc-518174 AF594 | 200 µg/ml | $357.00 | |||
5-LO Antibody (B-3) Alexa Fluor® 647 | sc-518174 AF647 | 200 µg/ml | $357.00 | |||
5-LO Antibody (B-3) Alexa Fluor® 680 | sc-518174 AF680 | 200 µg/ml | $357.00 | |||
5-LO Antibody (B-3) Alexa Fluor® 790 | sc-518174 AF790 | 200 µg/ml | $357.00 |