26S Proteasome p54 Antibody (28): sc-65748

26S Proteasome p54 Antibody (28) is a mouse monoclonal IgG2b kappa light chain antibody that detects 26S Proteasome p54 protein of Drosophila melanogaster origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and flow cytometry (FCM). Anti-26S Proteasome p54 antibody (28) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. The 26S proteasome is a crucial large complex responsible for intracellular degradation of proteins in eukaryotic cells, playing a vital role in maintaining cellular homeostasis and regulating numerous biological processes. This complex is composed of over 30 different subunits, with at least two copies of each, and assembly is ATP-dependent, which is essential for functionality. 26S proteasome p54, also known as Rpn10 in yeast and S5a in humans, is one of four non-ATPase base subunits of the 19S regulatory complex and is integral to peptidase activity of the proteasome. 26S proteasome p54 can bind to multiple ubiquitin chains, which is critical for recognizing and processing polyubiquitinated substrates targeted for degradation. In the presence of zinc, this subunit dissociates from the 19S complex, leading to a loss of peptidase activity, highlighting the importance of metal ions in regulating proteasome function. Upon dissociation, 26S Proteasome p54 interacts with non-proteasomal proteins such as HSP 82, Smt3, and UBC9, indicating involvement in various cellular pathways beyond protein degradation.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

26S Proteasome p54 Antibody (28) References:

1. Assembly of the Drosophila 26 S proteasome is accompanied by extensive subunit rearrangements.  |  Kurucz, E., et al. 2002. Biochem J. 365: 527-36. PMID: 11945175
2. A proteasomal ATPase subunit recognizes the polyubiquitin degradation signal.  |  Lam, YA., et al. 2002. Nature. 416: 763-7. PMID: 11961560
3. Tissue- and developmental stage-specific changes in the subcellular localization of the 26S proteasome in the ovary of Drosophila melanogaster.  |  Adám, G., et al. 2004. Gene Expr Patterns. 4: 329-33. PMID: 15053983
4. The HALTED ROOT gene encoding the 26S proteasome subunit RPT2a is essential for the maintenance of Arabidopsis meristems.  |  Ueda, M., et al. 2004. Development. 131: 2101-11. PMID: 15073153
5. ATP hydrolysis-dependent disassembly of the 26S proteasome is part of the catalytic cycle.  |  Babbitt, SE., et al. 2005. Cell. 121: 553-565. PMID: 15907469
6. The proteasome regulatory particle alters the SAGA coactivator to enhance its interactions with transcriptional activators.  |  Lee, D., et al. 2005. Cell. 123: 423-36. PMID: 16269334
7. The proteasome restricts permissive transcription at tissue-specific gene loci in embryonic stem cells.  |  Szutorisz, H., et al. 2006. Cell. 127: 1375-88. PMID: 17190601
8. Aging perturbs 26S proteasome assembly in Drosophila melanogaster.  |  Vernace, VA., et al. 2007. FASEB J. 21: 2672-82. PMID: 17413001
9. Rpn13p and Rpn14p are involved in the recognition of ubiquitinated Gcn4p by the 26S proteasome.  |  Seong, KM., et al. 2007. FEBS Lett. 581: 2567-73. PMID: 17499717

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-26S Proteasome p54 Antibody (28) has 3 citations in a variety of scientific publications.

Primary antibodies for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

26S Proteasome p54 Antibody (28) Specifications

Alternate Product Name: 26S Proteasome p54

Product Clone Name: 28

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids:

Epitope Target: 26S Proteasome

Epitope Species: Drosophila melanogaster

Product Reactivity/Detection: p54 subunit of the 19S regulatory base complex of the 26S Proteasome

Species Reactivity/Detection: d

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, FCM

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 3 citations in various scientific publications

Available Conjugates/Forms for 26S Proteasome p54 Antibody (28): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin