20S Proteasome β7 Antibody (H-3): sc-365725
- 20S Proteasome β7 Antibody (H-3) is a mouse monoclonal IgG2b κ, cited in 3 publications, provided at 200 µg/ml
- raised against amino acids 146-252 mapping within an internal region of 20S Proteasome β7 of human origin
- Anti-20S Proteasome beta 7 Antibody (H-3) is recommended for detection of 20S Proteasome β7 of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-20S Proteasome beta 7 Antibody (H-3) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG2b BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for 20S Proteasome β7 Antibody (H-3) for WB applications. These reagents are now offered in bundles with 20S Proteasome β7 Antibody (H-3) (see ordering information below).
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20S Proteasome β7 Antibody (H-3) is a mouse monoclonal IgG2b kappa light chain antibody that detects 20S Proteasome β7 (PSMB7) in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-PSMB7 antibody (H-3) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. PSMB7 plays a crucial role in cellular protein homeostasis by degrading unnecessary or damaged proteins, a process vital for maintaining cellular function and regulating various biological pathways. Located in the cytoplasm and nucleus, PSMB7 is a core component of the larger 26S proteasome complex, which is essential for the selective degradation of ubiquitin-tagged proteins. The structure of PSMB7 is composed of four stacked rings, with two outer rings made up of seven alpha subunits and two inner rings consisting of seven beta subunits, which are primarily responsible for the proteolytic activity. This unique arrangement not only facilitates the entry of substrate proteins but also ensures that the degradation process is tightly regulated, preventing uncontrolled proteolysis that could lead to cellular dysfunction.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
20S Proteasome β7 Antibody (H-3) References:
- Primary structural features of the 20S proteasome subunits of rice (Oryza sativa). | Sassa, H., et al. 2000. Gene. 250: 61-6. PMID: 10854779
- Catalytic site-specific inhibition of the 20S proteasome by 4-hydroxynonenal. | Ferrington, DA. and Kapphahn, RJ. 2004. FEBS Lett. 578: 217-23. PMID: 15589823
- Comprehensive mass spectrometric analysis of the 20S proteasome complex. | Huang, L. and Burlingame, AL. 2005. Methods Enzymol. 405: 187-236. PMID: 16413316
- Optimization of subsite binding to the beta5 subunit of the human 20S proteasome using vinyl sulfones and 2-keto-1,3,4-oxadiazoles: syntheses and cellular properties of potent, selective proteasome inhibitors. | Rydzewski, RM., et al. 2006. J Med Chem. 49: 2953-68. PMID: 16686537
- Role of the beta1 subunit in the function and stability of the 20S proteasome in the hyperthermophilic archaeon Pyrococcus furiosus. | Madding, LS., et al. 2007. J Bacteriol. 189: 583-90. PMID: 17114253
- Ordered structure of the crystallized bovine 20S proteasome. | Morimoto, Y., et al. 1995. J Biochem. 117: 471-4. PMID: 7629009
- Conformational constraints in protein degradation by the 20S proteasome. | Wenzel, T. and Baumeister, W. 1995. Nat Struct Biol. 2: 199-204. PMID: 7773788
- Human proteasome subunits from 2-dimensional gels identified by partial sequencing. | Kristensen, P., et al. 1994. Biochem Biophys Res Commun. 205: 1785-9. PMID: 7811265
- Structure and structure formation of the 20S proteasome. | Schmidt, M., et al. 1997. Mol Biol Rep. 24: 103-12. PMID: 9228290
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
20S Proteasome β7 Antibody (H-3) | sc-365725 | 200 µg/ml | $316.00 | |||
20S Proteasome β7 Antibody (H-3): m-IgG Fc BP-HRP Bundle | sc-529448 | 200 µg Ab; 10 µg BP | $354.00 | |||
20S Proteasome β7 Antibody (H-3): m-IgGκ BP-HRP Bundle | sc-522341 | 200 µg Ab, 40 µg BP | $354.00 | |||
20S Proteasome β7 Antibody (H-3): m-IgG2b BP-HRP Bundle | sc-550111 | 200 µg Ab; 10 µg BP | $354.00 | |||
20S Proteasome β7 Antibody (H-3) AC | sc-365725 AC | 500 µg/ml, 25% agarose | $416.00 | |||
20S Proteasome β7 Antibody (H-3) HRP | sc-365725 HRP | 200 µg/ml | $316.00 | |||
20S Proteasome β7 Antibody (H-3) FITC | sc-365725 FITC | 200 µg/ml | $330.00 | |||
20S Proteasome β7 Antibody (H-3) PE | sc-365725 PE | 200 µg/ml | $343.00 | |||
20S Proteasome β7 Antibody (H-3) Alexa Fluor® 488 | sc-365725 AF488 | 200 µg/ml | $357.00 | |||
20S Proteasome β7 Antibody (H-3) Alexa Fluor® 546 | sc-365725 AF546 | 200 µg/ml | $357.00 | |||
20S Proteasome β7 Antibody (H-3) Alexa Fluor® 594 | sc-365725 AF594 | 200 µg/ml | $357.00 | |||
20S Proteasome β7 Antibody (H-3) Alexa Fluor® 647 | sc-365725 AF647 | 200 µg/ml | $357.00 | |||
20S Proteasome β7 Antibody (H-3) Alexa Fluor® 680 | sc-365725 AF680 | 200 µg/ml | $357.00 | |||
20S Proteasome β7 Antibody (H-3) Alexa Fluor® 790 | sc-365725 AF790 | 200 µg/ml | $357.00 |